Visualizing lipid-formulated siRNA release from endosomes and target gene knockdown.

Research output: Contribution to journalArticle

Abstract

A central hurdle in developing small interfering RNAs (siRNAs) as therapeutics is the inefficiency of their delivery across the plasma and endosomal membranes to the cytosol, where they interact with the RNA interference machinery. With the aim of improving endosomal release, a poorly understood and inefficient process, we studied the uptake and cytosolic release of siRNAs, formulated in lipoplexes or lipid nanoparticles, by live-cell imaging and correlated it with knockdown of a target GFP reporter. siRNA release occurred invariably from maturing endosomes within ∼5-15 min of endocytosis. Cytosolic galectins immediately recognized the damaged endosome and targeted it for autophagy. However, inhibiting autophagy did not enhance cytosolic siRNA release. Gene knockdown occurred within a few hours of release and required <2,000 copies of cytosolic siRNAs. The ability to detect cytosolic release of siRNAs and understand how it is regulated will facilitate the development of rational strategies for improving the cytosolic delivery of candidate drugs.

Details

Authors
  • Anders Wittrup
  • Angela Ai
  • Xing Liu
  • Peter Hamar
  • Radiana Trifonova
  • Klaus Charisse
  • Muthiah Manoharan
  • Tomas Kirchhausen
  • Judy Lieberman
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Microbiology in the medical area
Original languageEnglish
Pages (from-to)870
JournalNature Biotechnology
Volume33
Issue number8
Publication statusPublished - 2015
Publication categoryResearch
Peer-reviewedYes