Vitamin D deficiency and ovariectomy reduced the strength of the femoral neck in rats

Research output: Contribution to journalArticle

Standard

Vitamin D deficiency and ovariectomy reduced the strength of the femoral neck in rats. / Kaastad, T S; Reikeras, O; Halvorsen, V; Falch, J A; Obrant, Karl; Nordsletten, L.

In: Calcified Tissue International, Vol. 69, No. 2, 2001, p. 102-108.

Research output: Contribution to journalArticle

Harvard

Kaastad, TS, Reikeras, O, Halvorsen, V, Falch, JA, Obrant, K & Nordsletten, L 2001, 'Vitamin D deficiency and ovariectomy reduced the strength of the femoral neck in rats', Calcified Tissue International, vol. 69, no. 2, pp. 102-108. https://doi.org/10.1007/s00223-001-0009-2

APA

Kaastad, T. S., Reikeras, O., Halvorsen, V., Falch, J. A., Obrant, K., & Nordsletten, L. (2001). Vitamin D deficiency and ovariectomy reduced the strength of the femoral neck in rats. Calcified Tissue International, 69(2), 102-108. https://doi.org/10.1007/s00223-001-0009-2

CBE

MLA

Vancouver

Author

Kaastad, T S ; Reikeras, O ; Halvorsen, V ; Falch, J A ; Obrant, Karl ; Nordsletten, L. / Vitamin D deficiency and ovariectomy reduced the strength of the femoral neck in rats. In: Calcified Tissue International. 2001 ; Vol. 69, No. 2. pp. 102-108.

RIS

TY - JOUR

T1 - Vitamin D deficiency and ovariectomy reduced the strength of the femoral neck in rats

AU - Kaastad, T S

AU - Reikeras, O

AU - Halvorsen, V

AU - Falch, J A

AU - Obrant, Karl

AU - Nordsletten, L

PY - 2001

Y1 - 2001

N2 - Vitamin D (vit D) deficiency is common in the elderly, and the aim of this study was to investigate whether vit D deprivation in ovariectomized (ovx) and normal rats would reduce fracture strength. Forty mature female Wistar rats were randomized into four groups: two were ovariectomized (ovx) and two were sham-operated (sham). One ovx and one sham group were fed a vit D-deficient diet (Ovx-D and Sham-D), and the control groups were fed normal rat chow (Ovx and Sham) for 12 weeks. Vit D deficiency was substantiated after 12 weeks by undetectable serum concentrations of 25OHD in the Sham-D and Ovx-D groups. 85Sr activity was lower in Sham-D than in the other groups (P < 0.005). Tibial and femoral weights and lengths showed no differences. Distal tibial trabecular bone volume was reduced in both ovx groups compare with sham (P < 0.005). Bone mineral density (BMD) was higher in sham than in Sham-D and both ovx groups (P < 0.005). Femoral area moment of inertia increased and ultimate stress decreased in Ovx-D compared with ovx (P < 0.05). Other biomechanical properties of the femoral shafts did not differ significantly. The femoral neck was significantly weaker in Ovx-D than in the other groups. In conclusion, ovx decreased tibial trabecular bone volume and both ovx and vit D depletion reduced femoral BMD in rats. Vit D depletion reduced the ultimate stress in the femoral shaft, and the combined depletion of estrogen and vit D significantly reduced the fracture strength in the femoral neck. This fits well with clinical evidence of how postmenopausal status combined with vit D deficiency lead to an increased risk of hip fractures, making this animal model a possible tool for investigating measures to prevent such fractures.

AB - Vitamin D (vit D) deficiency is common in the elderly, and the aim of this study was to investigate whether vit D deprivation in ovariectomized (ovx) and normal rats would reduce fracture strength. Forty mature female Wistar rats were randomized into four groups: two were ovariectomized (ovx) and two were sham-operated (sham). One ovx and one sham group were fed a vit D-deficient diet (Ovx-D and Sham-D), and the control groups were fed normal rat chow (Ovx and Sham) for 12 weeks. Vit D deficiency was substantiated after 12 weeks by undetectable serum concentrations of 25OHD in the Sham-D and Ovx-D groups. 85Sr activity was lower in Sham-D than in the other groups (P < 0.005). Tibial and femoral weights and lengths showed no differences. Distal tibial trabecular bone volume was reduced in both ovx groups compare with sham (P < 0.005). Bone mineral density (BMD) was higher in sham than in Sham-D and both ovx groups (P < 0.005). Femoral area moment of inertia increased and ultimate stress decreased in Ovx-D compared with ovx (P < 0.05). Other biomechanical properties of the femoral shafts did not differ significantly. The femoral neck was significantly weaker in Ovx-D than in the other groups. In conclusion, ovx decreased tibial trabecular bone volume and both ovx and vit D depletion reduced femoral BMD in rats. Vit D depletion reduced the ultimate stress in the femoral shaft, and the combined depletion of estrogen and vit D significantly reduced the fracture strength in the femoral neck. This fits well with clinical evidence of how postmenopausal status combined with vit D deficiency lead to an increased risk of hip fractures, making this animal model a possible tool for investigating measures to prevent such fractures.

U2 - 10.1007/s00223-001-0009-2

DO - 10.1007/s00223-001-0009-2

M3 - Article

VL - 69

SP - 102

EP - 108

JO - Calcified Tissue International

JF - Calcified Tissue International

SN - 1432-0827

IS - 2

ER -