A microfluidic analysis system for an in vitro model of the blood-brain barrier

Projekt: AvhandlingTvärvetenskaplig forskning



Populärvetenskaplig beskrivning

Animal models are widely used in research and drug development today, but they are not analogous to the human physiology, and often fail to predict drug efficacy and human in vivo responses. Using animal models is a source of ethical concern as many experiments cause the animals pain, suffering, or general distress. With this in mind, development of new and improved in vitro models is of great interest.
This licentiate thesis has in vitro device development as its main focus. The the- sis comprises one paper describing the method of creating a microfluidic cell culture device with integrated sensors and excellent monitoring possibilities. The device is created using thin-film techniques for metal deposition, as well as optimized assem- bly procedures, cell culture methods and microfluidic flow protocols. The system is revolutionary, as provides a solution for three different challenges in the field of organs-on-chip, namely: visibility, electrical monitoring, and flow inclusion.
In addition, this thesis contains two peer-reviewed conference proceedings, de- tailing a novel seeding technique for microfluidic systems, and the optimization of the bonding protocols of our microfluidic system respectively.
Kort titelA microfluidic analysis system for an in vitro model of the blood-brain barrier
Gällande start-/slutdatum2015/01/052019/06/17