100% complete assignment of non-labile H-1, C-13, and N-15 signals for calcium-loaded calbindin D-9k P43G

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Here we present the 100% complete assignment chemical shift of non-labile H-1, N-15 and C-13 nuclei of Calbindin D-9k P43G. The assignment includes all non-exchangeable side chain nuclei, including ones that are rarely reported, such as LysN zeta as well as the termini. NMR experiments required to achieve truly complete assignments are discussed. To the best of our knowledge our assignments for Calbindin D-9k extend beyond previous studies reaching near-completeness (Vis et al. in Biochem 33:14858-14870, 1994; Yamazaki et al. in J Am Chem Soc 116:6464-6465, 1994; Yamazaki et al. in Biochem 32:5656-5669, 1993b).

Detaljer

Författare
  • Nur Alia Oktaviani
  • Renee Otten
  • Klaas Dijkstra
  • Ruud M. Scheek
  • Eva Thulin
  • Mikael Akke
  • Frans Mulder
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Fysikalisk kemi

Nyckelord

Originalspråkengelska
Sidor (från-till)79-84
TidskriftBiomolecular NMR Assignments
Volym5
Utgåva nummer1
StatusPublished - 2011
PublikationskategoriForskning
Peer review utfördJa