140 mouse brain proteins identified by Ca2+-calmodulin affinity chromatography and tandem mass spectrometry.

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title = "140 mouse brain proteins identified by Ca2+-calmodulin affinity chromatography and tandem mass spectrometry.",
abstract = "Calmodulin is an essential Ca2+-binding protein that binds to a variety of targets that carry out critical signaling functions. We describe the proteomic characterization of mouse brain Ca2+-calmodulin-binding proteins that were purified using calmodulin affinity chromatography. Proteins in the eluates from four different affinity chromatography experiments were identified by 1-DE and in-gel digestion followed by LC-MS/MS. Parallel experiments were performed using two related control-proteins belonging to the EF-hand family. After comparing the results from the different experiments, we were able to exclude a significant number of proteins suspected to bind in a nonspecific manner. A total of 140 putative Ca2+-calmodulin-binding proteins were identified of which 87 proteins contained calmodulin-binding motifs. Among the 87 proteins that contained calmodulin-binding motifs, 48 proteins have not previously been shown to interact with calmodulin and 39 proteins were known calmodulin-binding proteins. Many proteins with ill-defined functions were identified as well as a number of proteins that at the time of the analysis were described only as ORFs. This study provides a functional framework for studies on these previously uncharacterized proteins.",
keywords = "calcium, protein-protein interactions, calmodulin, proteomics, brain",
author = "Tord Bergg{\aa}rd and Giorgio Arrigoni and Olof Olsson and Malin Fex and Sara Linse and Peter James",
year = "2006",
doi = "10.1021/pr050421l",
language = "English",
volume = "5",
pages = "669--687",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "The American Chemical Society (ACS)",
number = "3",