14-3-3 expression in denervated hippocampus after entorhinal cortex lesion assessed by culture-derived isotope tags in quantitative proteomics

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Activation of astrocytes accompanies many brain pathologies. Reactive astrocytes have a beneficial role in acute neurotrauma but later on might inhibit regeneration. 2D-gel electrophoresis and mass spectrometry were applied to study the proteome difference in denervated hippocampus in wildtype mice and mice lacking intermediate filament proteins glial fibrillary acidic protein (GFAP) and vimentin (GFAP-/-Vim-/-) that show attenuated reactive gliosis and enhanced posttraumatic regeneration. Proteomic data and immunohistochemical analyses showed upregulation of the adapter protein 14-3-3 four days postlesion and suggested that 14-3-3 upregulation after injury is triggered by reactive gliosis. Culture-derived isotope tags (CDIT) and mass spectrometry demonstrated that 14-3-3 epsilon was the major isoform upregulated in denervated hippocampus and that its upregulation was attenuated in GFAP-/-Vim-/- mice and thus most likely connected to reactive gliosis.

Detaljer

Författare
Externa organisationer
  • Florida State University
Forskningsområden

Nyckelord

Originalspråkengelska
Sidor (från-till)3491-3500
Antal sidor10
TidskriftJournal of Proteome Research
Volym6
Utgivningsnummer9
StatusPublished - 2007 sep
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa