2D polarization imaging as a low-cost fluorescence method to detect α-synuclein aggregation ex vivo in models of Parkinson’s disease

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

A hallmark of Parkinson’s disease is the formation of large protein-rich aggregates in neurons, where α-synuclein is the most abundant protein. A standard approach to visualize aggregation is to fluorescently label the proteins of interest. Then, highly fluorescent regions are assumed to contain aggregated proteins. However, fluorescence brightness alone cannot discriminate micrometer-sized regions with high expression of non-aggregated proteins from regions where the proteins are aggregated on the molecular scale. Here, we demonstrate that 2-dimensional polarization imaging can discriminate between preformed non-aggregated and aggregated forms of α-synuclein, and detect increased aggregation in brain tissues of transgenic mice. This imaging method assesses homo-FRET between labels by measuring fluorescence polarization in excitation and emission simultaneously, which translates into higher contrast than fluorescence anisotropy imaging. Exploring earlier aggregation states of α-synuclein using such technically simple imaging method could lead to crucial improvements in our understanding of α-synuclein-mediated pathology in Parkinson’s Disease.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • Leibniz Institute of Photonic Technology (IPHT)
  • China Medical University, Shenyang
  • François Jacob Institute of biology
  • Friedrich Schiller University Jena
  • Lund University
  • Catholic University of Leuven
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biofysik
  • Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)
  • Biokemi och molekylärbiologi
  • Klinisk laboratoriemedicin
Originalspråkengelska
Artikelnummer157
TidskriftCommunications Biology
Volym1
StatusPublished - 2018
PublikationskategoriForskning
Peer review utfördJa