3D MAS NMR Experiment Utilizing Through-Space 15N-15N Correlations

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

We demonstrate a novel 3D NNC magic angle spinning NMR experiment that generates 15N-15N internuclear contacts in protein systems using an optimized 15N-15N proton assisted recoupling (PAR) mixing period and a 13C dimension for improved resolution. The optimized PAR condition permits the acquisition of high signal-to-noise 3D data that enables backbone chemical shift assignments using a strategy that is complementary to current schemes. The spectra can also provide distance constraints. The utility of the experiment is demonstrated on an M01-42 fibril sample that yields high-quality data that is readily assigned and interpreted. The 3D NNC experiment therefore provides a powerful platform for solid-state protein studies and is broadly applicable to a variety of systems and experimental conditions.

Detaljer

Författare
  • Kevin J. Donovan
  • Robert Silvers
  • Sara Linse
  • Robert G Griffin
Enheter & grupper
Externa organisationer
  • Massachusetts Institute of Technology
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biokemi och molekylärbiologi
Originalspråkengelska
Sidor (från-till)6518-6521
Antal sidor4
TidskriftJournal of the American Chemical Society
Volym139
Utgivningsnummer19
StatusPublished - 2017 maj 17
PublikationskategoriForskning
Peer review utfördJa