3He MRI-based assessment of posture-dependent regional ventilation gradients in rats

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

A recently developed method for quantitative assessment of regional lung ventilation was employed for the study of posture-dependent ventilation differences in rats. The measurement employed hyperpolarized (3)He MRI to detect the build-up of the signal intensity after increasing numbers of (3)He breaths, which allowed for computation of a regional ventilation parameter. A group of six anesthetized rats was studied in both supine and prone postures. Three-dimensional maps of the ventilation parameter were obtained with high spatial resolution (voxel volume approximately 2 mm(3)). Vertical (dorsal-ventral) gradients of the ventilation index, defined as the regional ventilation normalized by the average ventilation within the whole lung, were investigated. Variations in the regional distribution of the ventilation parameter, as well as of the ventilation index, could be detected, depending on the posture of the rats. In supine posture, ventilation was elevated in the dependent parts of the lungs, with a linear gradient of the ventilation index of -0.11 +/- 0.03 cm(-1). In prone posture, the distribution of ventilation was more uniform, with a significantly (P < 0.001) smaller gradient of the ventilation index of -0.01 +/- 0.02 cm(-1). It is concluded that the (3)He MRI-based method can detect and quantify regional ventilation gradients in animals as small as the rat and that these gradients depend on prone or supine posture of the animal.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • Skåne University Hospital
  • Amersham Health R and D, Malmö
Forskningsområden

Nyckelord

Originalspråkengelska
Sidor (från-till)2259-67
Antal sidor9
TidskriftJournal of Applied Physiology
Volym98
Utgivningsnummer6
StatusPublished - 2005 jun
PublikationskategoriForskning
Peer review utfördJa