3-Hydroxy fatty acids in saliva as diagnostic markers in chronic periodontitis

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3-Hydroxy fatty acids in saliva as diagnostic markers in chronic periodontitis. / Ferrando, R; Szponar, Bogumila; Sanchez, A; Larsson, Lennart; Valero-Guillen, P L.

I: Journal of Microbiological Methods, Vol. 62, Nr. 3, 2005, s. 285-291.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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Ferrando, R ; Szponar, Bogumila ; Sanchez, A ; Larsson, Lennart ; Valero-Guillen, P L. / 3-Hydroxy fatty acids in saliva as diagnostic markers in chronic periodontitis. I: Journal of Microbiological Methods. 2005 ; Vol. 62, Nr. 3. s. 285-291.

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TY - JOUR

T1 - 3-Hydroxy fatty acids in saliva as diagnostic markers in chronic periodontitis

AU - Ferrando, R

AU - Szponar, Bogumila

AU - Sanchez, A

AU - Larsson, Lennart

AU - Valero-Guillen, P L

PY - 2005

Y1 - 2005

N2 - Saturated straight- and branched-chain 3-hydroxy fatty acids (3-OH FAs) of 10-18 carbon chain lengths were determined in saliva from 27 individuals with chronic periodontitis and 18 healthy individuals by using gas chromatography-tandem mass spectrometry. Of the 14 different 3-OH FAs detected, 3-OH-C-i17:0 was the most abundant in the periodontitis samples while 3-OH-C-14:0 was the most abundant in the healthy individuals. Considering the relative percentages of 3-OH-C-12:0, 3-OH-C-14:0, 3OH-C-i17:0, and 3-OH-C-17:0, 95.6% of all cases were correctly classified as healthy individuals or periodontitis patients by means of discriminant analysis. The sensitivity, specificity, positive predictive value and negative predictive value of 3-OH FA analysis in diagnosing peridontitis were, respectively, 0.92, 1.00, 1.00, and 0.90. The results indicate that 3-OH FA analysis of saliva samples is a useful diagnostic method in chronic periodontitis.

AB - Saturated straight- and branched-chain 3-hydroxy fatty acids (3-OH FAs) of 10-18 carbon chain lengths were determined in saliva from 27 individuals with chronic periodontitis and 18 healthy individuals by using gas chromatography-tandem mass spectrometry. Of the 14 different 3-OH FAs detected, 3-OH-C-i17:0 was the most abundant in the periodontitis samples while 3-OH-C-14:0 was the most abundant in the healthy individuals. Considering the relative percentages of 3-OH-C-12:0, 3-OH-C-14:0, 3OH-C-i17:0, and 3-OH-C-17:0, 95.6% of all cases were correctly classified as healthy individuals or periodontitis patients by means of discriminant analysis. The sensitivity, specificity, positive predictive value and negative predictive value of 3-OH FA analysis in diagnosing peridontitis were, respectively, 0.92, 1.00, 1.00, and 0.90. The results indicate that 3-OH FA analysis of saliva samples is a useful diagnostic method in chronic periodontitis.

KW - gas chronnatography-mass spectrometry

KW - acids

KW - anaerobic gram-negative bacteria/periodontal pathogens

KW - 3-hydroxy fatty

KW - periodontitis/etiology

KW - microbial chemical

KW - inarkers

U2 - 10.1016/j.mimet.2005.04.014

DO - 10.1016/j.mimet.2005.04.014

M3 - Article

VL - 62

SP - 285

EP - 291

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

SN - 1872-8359

IS - 3

ER -