A 7-gene signature depicts the biochemical profile of early prefibrotic myelofibrosis

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A 7-gene signature depicts the biochemical profile of early prefibrotic myelofibrosis. / Skov, Vibe; Burton, Mark; Thomassen, Mads; Larsen, Thomas Stauffer; Riley, Caroline H.; Madelung, Ann Brinch; Kjær, Lasse; Bondo, Henrik; Stamp, Inger; Ehinger, Mats; Dahl-Sørensen, Rasmus; Brochmann, Nana; Nielsen, Karsten; Thiele, Jürgen; Jensen, Morten K.; Bjerrum, Ole Weis; Kruse, Torben A.; Hasselbalch, Hans Carl.

I: PLoS ONE, Vol. 11, Nr. 8, e0161570, 01.08.2016.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Skov, V, Burton, M, Thomassen, M, Larsen, TS, Riley, CH, Madelung, AB, Kjær, L, Bondo, H, Stamp, I, Ehinger, M, Dahl-Sørensen, R, Brochmann, N, Nielsen, K, Thiele, J, Jensen, MK, Bjerrum, OW, Kruse, TA & Hasselbalch, HC 2016, 'A 7-gene signature depicts the biochemical profile of early prefibrotic myelofibrosis', PLoS ONE, vol. 11, nr. 8, e0161570. https://doi.org/10.1371/journal.pone.0161570

APA

Skov, V., Burton, M., Thomassen, M., Larsen, T. S., Riley, C. H., Madelung, A. B., Kjær, L., Bondo, H., Stamp, I., Ehinger, M., Dahl-Sørensen, R., Brochmann, N., Nielsen, K., Thiele, J., Jensen, M. K., Bjerrum, O. W., Kruse, T. A., & Hasselbalch, H. C. (2016). A 7-gene signature depicts the biochemical profile of early prefibrotic myelofibrosis. PLoS ONE, 11(8), [e0161570]. https://doi.org/10.1371/journal.pone.0161570

CBE

Skov V, Burton M, Thomassen M, Larsen TS, Riley CH, Madelung AB, Kjær L, Bondo H, Stamp I, Ehinger M, Dahl-Sørensen R, Brochmann N, Nielsen K, Thiele J, Jensen MK, Bjerrum OW, Kruse TA, Hasselbalch HC. 2016. A 7-gene signature depicts the biochemical profile of early prefibrotic myelofibrosis. PLoS ONE. 11(8):Article e0161570. https://doi.org/10.1371/journal.pone.0161570

MLA

Vancouver

Skov V, Burton M, Thomassen M, Larsen TS, Riley CH, Madelung AB et al. A 7-gene signature depicts the biochemical profile of early prefibrotic myelofibrosis. PLoS ONE. 2016 aug 1;11(8). e0161570. https://doi.org/10.1371/journal.pone.0161570

Author

Skov, Vibe ; Burton, Mark ; Thomassen, Mads ; Larsen, Thomas Stauffer ; Riley, Caroline H. ; Madelung, Ann Brinch ; Kjær, Lasse ; Bondo, Henrik ; Stamp, Inger ; Ehinger, Mats ; Dahl-Sørensen, Rasmus ; Brochmann, Nana ; Nielsen, Karsten ; Thiele, Jürgen ; Jensen, Morten K. ; Bjerrum, Ole Weis ; Kruse, Torben A. ; Hasselbalch, Hans Carl. / A 7-gene signature depicts the biochemical profile of early prefibrotic myelofibrosis. I: PLoS ONE. 2016 ; Vol. 11, Nr. 8.

RIS

TY - JOUR

T1 - A 7-gene signature depicts the biochemical profile of early prefibrotic myelofibrosis

AU - Skov, Vibe

AU - Burton, Mark

AU - Thomassen, Mads

AU - Larsen, Thomas Stauffer

AU - Riley, Caroline H.

AU - Madelung, Ann Brinch

AU - Kjær, Lasse

AU - Bondo, Henrik

AU - Stamp, Inger

AU - Ehinger, Mats

AU - Dahl-Sørensen, Rasmus

AU - Brochmann, Nana

AU - Nielsen, Karsten

AU - Thiele, Jürgen

AU - Jensen, Morten K.

AU - Bjerrum, Ole Weis

AU - Kruse, Torben A.

AU - Hasselbalch, Hans Carl

PY - 2016/8/1

Y1 - 2016/8/1

N2 - Recent studies have shown that a large proportion of patients classified as essential thrombocythemia (ET) actually have early primary prefibrotic myelofibrosis (prePMF), which implies an inferior prognosis as compared to patients being diagnosed with so-called genuine or true ET. According to theWorld Health Organization (WHO) 2008 classification, bone marrow histology is a major component in the distinction between these disease entities. However, the differential diagnosis between themmay be challenging and several studies have not been able to distinguish between them.Most lately, it has been argued that simple blood tests, including the leukocyte count and plasma lactate dehydrogenase (LDH) may be useful tools to separate genuine ET from prePMF, the latter disease entity more often being featured by anemia, leukocytosis and elevated LDH.Whole blood gene expression profiling was performed in 17 and 9 patients diagnosed with ET and PMF, respectively. Using elevated LDH obtained at the time of diagnosis as a marker of prePMF, a 7-gene signature was identified which correctly predicted the prePMF group with a sensitivity of 100%and a specificity of 89%. The 7 genes included MPO, CEACAM8, CRISP3, MS4A3, CEACAM6, HEMGN, andMMP8, which are genes known to be involved in inflammation, cell adhesion, differentiation and proliferation. Evaluation of bone marrow biopsies and the 7-gene signature showed a concordance rate of 71%, 79%, 62%, and 38%. Our 7-gene signature may be a useful tool to differentiate between genuine ET and prePMF but needs to be validated in a larger cohort of "ET" patients.

AB - Recent studies have shown that a large proportion of patients classified as essential thrombocythemia (ET) actually have early primary prefibrotic myelofibrosis (prePMF), which implies an inferior prognosis as compared to patients being diagnosed with so-called genuine or true ET. According to theWorld Health Organization (WHO) 2008 classification, bone marrow histology is a major component in the distinction between these disease entities. However, the differential diagnosis between themmay be challenging and several studies have not been able to distinguish between them.Most lately, it has been argued that simple blood tests, including the leukocyte count and plasma lactate dehydrogenase (LDH) may be useful tools to separate genuine ET from prePMF, the latter disease entity more often being featured by anemia, leukocytosis and elevated LDH.Whole blood gene expression profiling was performed in 17 and 9 patients diagnosed with ET and PMF, respectively. Using elevated LDH obtained at the time of diagnosis as a marker of prePMF, a 7-gene signature was identified which correctly predicted the prePMF group with a sensitivity of 100%and a specificity of 89%. The 7 genes included MPO, CEACAM8, CRISP3, MS4A3, CEACAM6, HEMGN, andMMP8, which are genes known to be involved in inflammation, cell adhesion, differentiation and proliferation. Evaluation of bone marrow biopsies and the 7-gene signature showed a concordance rate of 71%, 79%, 62%, and 38%. Our 7-gene signature may be a useful tool to differentiate between genuine ET and prePMF but needs to be validated in a larger cohort of "ET" patients.

UR - http://www.scopus.com/inward/record.url?scp=84991492547&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0161570

DO - 10.1371/journal.pone.0161570

M3 - Article

C2 - 27579896

AN - SCOPUS:84991492547

VL - 11

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 8

M1 - e0161570

ER -