A Biomimetic, Copolymeric Membrane for Cell-Stretch Experiments with Pulmonary Epithelial Cells at the Air-Liquid Interface

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Chronic respiratory diseases are among the leading causes of death worldwide, but only symptomatic therapies are available for terminal illness. This in part reflects a lack of biomimetic in vitro models that can imitate the complex environment and physiology of the lung. Here, a copolymeric membrane consisting of poly(ε-)caprolactone and gelatin with tunable properties, resembling the main characteristics of the alveolar basement membrane is introduced. The thin bioinspired membrane (0.5 μm) is stretchable (up to 25% linear strain) with appropriate surface wettability and porosity for culturing lung epithelial cells under air–liquid interface conditions. The unique biphasic concept of this membrane provides optimum characteristics for initial cell growth (phase I) and then switch to biomimetic properties for cyclic cell-stretch experiments (phase II). It is showed that physiologic cyclic mechanical stretch improves formation of F-actin cytoskeleton filaments and tight junctions while non-physiologic over-stretch induces cell apoptosis, activates inflammatory response (IL-8), and impairs epithelial barrier integrity. It is also demonstrated that cyclic physiologic stretch can enhance the cellular uptake of nanoparticles. Since this membrane offers considerable advantages over currently used membranes, it may lead the way to more biomimetic in vitro models of the lung for translation of in vitro response studies into clinical outcome.

Detaljer

Författare
  • Ali Doryab
  • Mehmet Berat Taskin
  • Philipp Stahlhut
  • Andreas Schröppel
  • Darcy E. Wagner
  • Jürgen Groll
  • Otmar Schmid
Enheter & grupper
Externa organisationer
  • German Center for Lung Research (DZL)
  • Helmholtz Zentrum München
  • Julius Maximilian University of Würzburg
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Lungmedicin och allergi

Nyckelord

Originalspråkengelska
Artikelnummer2004707
TidskriftAdvanced Functional Materials
Volym31
Utgåva nummer10
StatusPublished - 2021
PublikationskategoriForskning
Peer review utfördJa