A cultivation technique for E-coli fed-batch cultivations operating close to the maximum oxygen transfer capacity of the reactor

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A cultivation technique for E-coli fed-batch cultivations operating close to the maximum oxygen transfer capacity of the reactor. / de Maré, Lena; Velut, Stéphane; Ledung, E; Cimander, C; Norrman, B; Nordberg Karlsson, Eva; Holst, Olle; Hagander, Per.

I: Biotechnology Letters, Vol. 27, Nr. 14, 2005, s. 983-990.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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T1 - A cultivation technique for E-coli fed-batch cultivations operating close to the maximum oxygen transfer capacity of the reactor

AU - de Maré, Lena

AU - Velut, Stéphane

AU - Ledung, E

AU - Cimander, C

AU - Norrman, B

AU - Nordberg Karlsson, Eva

AU - Holst, Olle

AU - Hagander, Per

PY - 2005

Y1 - 2005

N2 - A cultivation strategy combining the advantages of temperature-limited fed-batch and probing feeding control is presented. The technique was evaluated in fed-batch cultivations with E. coli BL21(DE3) producing xylanase in a 3 liter bioreactor. A 20% increase in cell mass was achieved and the usual decrease in specific enzyme activity normally observed during the late production phase was diminished with the new technique. The method was further tested by growing E. coli W3110 in a larger bioreactor (50 l). It is a suitable cultivation technique when the O-2 transfer capacity of the reactor is reached and it is desired to continue to produce the recombinant protein.

AB - A cultivation strategy combining the advantages of temperature-limited fed-batch and probing feeding control is presented. The technique was evaluated in fed-batch cultivations with E. coli BL21(DE3) producing xylanase in a 3 liter bioreactor. A 20% increase in cell mass was achieved and the usual decrease in specific enzyme activity normally observed during the late production phase was diminished with the new technique. The method was further tested by growing E. coli W3110 in a larger bioreactor (50 l). It is a suitable cultivation technique when the O-2 transfer capacity of the reactor is reached and it is desired to continue to produce the recombinant protein.

U2 - 10.1007/s10529-005-7844-6

DO - 10.1007/s10529-005-7844-6

M3 - Article

VL - 27

SP - 983

EP - 990

JO - Biotechnology Letters

T2 - Biotechnology Letters

JF - Biotechnology Letters

SN - 0141-5492

IS - 14

ER -