A fibrin biofilm covers blood clots and protects from microbial invasion

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Hemostasis requires conversion of fibrinogen to fibrin fibers that generate a characteristic network, interact with blood cells, and initiate tissue repair. The fibrin network is porous and highly permeable, but the spatial arrangement of the external clot face is unknown. Here we show that fibrin transitioned to the blood-air interface through Langmuir film formation, producing a protective film confining clots in human and mouse models. We demonstrated that only fibrin is required for formation of the film, and that it occurred in vitro and in vivo. The fibrin film connected to the underlying clot network through tethering fibers. It was digested by plasmin, and formation of the film was prevented with surfactants. Functionally, the film retained blood cells and protected against penetration by bacterial pathogens in a murine model of dermal infection. Our data show a remarkable aspect of blood clotting in which fibrin forms a protective film covering the external surface of the clot, defending the organism against microbial invasion.

Detaljer

Författare
  • Fraser L. Macrae
  • Cédric Duval
  • Praveen Papareddy
  • Stephen R. Baker
  • Nadira Yuldasheva
  • Katherine J. Kearney
  • Helen R. McPherson
  • Nathan Asquith
  • Joke Konings
  • Alessandro Casini
  • Jay L. Degen
  • Simon D. Connell
  • Helen Philippou
  • Alisa S. Wolberg
  • Heiko Herwald
  • Robert A.S. Ariëns
Enheter & grupper
Externa organisationer
  • University of Leeds
  • Maastricht University Medical Centre
  • Geneva University Hospital
  • Cincinnati Children's Hospital Medical Center
  • University of North Carolina
  • Maastricht University
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Mikrobiologi inom det medicinska området
  • Hematologi
Originalspråkengelska
Sidor (från-till)3356-3368
Antal sidor13
TidskriftJournal of Clinical Investigation
Volym128
Utgivningsnummer8
StatusPublished - 2018 aug 1
PublikationskategoriForskning
Peer review utfördJa