A high-beef diet alter protein kinase C isozyme expression in rat colonic mucosa.

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title = "A high-beef diet alter protein kinase C isozyme expression in rat colonic mucosa.",
abstract = "We recently reported that a red meat (beef) diet relative to a casein-based diet increases protein kinase C (PKC) activity in rat colonic mucosa. The purpose of this study was to further elucidate the effects of a high-beef diet on colonic intracellular signal transduction by analyzing steady-state protein levels of different PKC isozymes as well as activities of the three types of sphingomyelinases. Male Wistar rats (n = 12/group) were fed AIN93G-based diets either high in beef or casein for 4 weeks. Rats fed the beef diet had significantly (P < 0.05) higher cytosolic PKC α and lower membrane PKC δ protein levels than rats fed the casein diet. The beef-fed rats also had alterations in subfractions of PKC ζ/λ so that they had a significantly (P = 0.001) lower level of membrane 70 & 75 kDa fraction and a higher (P = 0.001) level of cytosolic 40 & 43 kDa fraction than rats fed the casein diet. Because protein levels analyzed with a PKC ζ-specific antibody were similar, these differences in PKC ζ/λ were probably due to changes in PKC λ expression. PKC β2 levels did not differ between the dietary groups. Diet had no significant effect on the activity of acid, neutral, or alkaline sphingomyelinase. This study demonstrated that consumption of a high-beef diet is capable of modulating PKC isozyme levels in rat colon, which might be one of the mechanisms whereby red meat affects colon carcinogenesis.",
author = "Anne-Maria Pajari and SI Oikarinen and Rui-Dong Duan and Marja Mutanen",
year = "2000",
doi = "10.1016/S0955-2863(00)00100-5",
language = "English",
volume = "11",
pages = "474--481",
journal = "Nutrition Reports International",
issn = "1873-4847",
publisher = "Elsevier",
number = "10",