A high-quality catalog of the Drosophila melanogaster proteome

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Standard

A high-quality catalog of the Drosophila melanogaster proteome. / Brunner, Erich; Ahrens, Christian H; Mohanty, Sonali; Baetschmann, Hansruedi; Loevenich, Sandra; Potthast, Frank; Deutsch, Eric W; Panse, Christian; de Lichtenberg, Ulrik; Rinner, Oliver; Lee, Hookeun; Pedrioli, Patrick G A; Malmstrom, Johan; Koehler, Katja; Schrimpf, Sabine; Krijgsveld, Jeroen; Kregenow, Floyd; Heck, Albert J R; Hafen, Ernst; Schlapbach, Ralph; Aebersold, Ruedi.

I: Nature Biotechnology, Vol. 25, Nr. 5, 05.2007, s. 576-83.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Brunner, E, Ahrens, CH, Mohanty, S, Baetschmann, H, Loevenich, S, Potthast, F, Deutsch, EW, Panse, C, de Lichtenberg, U, Rinner, O, Lee, H, Pedrioli, PGA, Malmstrom, J, Koehler, K, Schrimpf, S, Krijgsveld, J, Kregenow, F, Heck, AJR, Hafen, E, Schlapbach, R & Aebersold, R 2007, 'A high-quality catalog of the Drosophila melanogaster proteome', Nature Biotechnology, vol. 25, nr. 5, s. 576-83. https://doi.org/10.1038/nbt1300

APA

Brunner, E., Ahrens, C. H., Mohanty, S., Baetschmann, H., Loevenich, S., Potthast, F., ... Aebersold, R. (2007). A high-quality catalog of the Drosophila melanogaster proteome. Nature Biotechnology, 25(5), 576-83. https://doi.org/10.1038/nbt1300

CBE

Brunner E, Ahrens CH, Mohanty S, Baetschmann H, Loevenich S, Potthast F, Deutsch EW, Panse C, de Lichtenberg U, Rinner O, Lee H, Pedrioli PGA, Malmstrom J, Koehler K, Schrimpf S, Krijgsveld J, Kregenow F, Heck AJR, Hafen E, Schlapbach R, Aebersold R. 2007. A high-quality catalog of the Drosophila melanogaster proteome. Nature Biotechnology. 25(5):576-83. https://doi.org/10.1038/nbt1300

MLA

Vancouver

Brunner E, Ahrens CH, Mohanty S, Baetschmann H, Loevenich S, Potthast F et al. A high-quality catalog of the Drosophila melanogaster proteome. Nature Biotechnology. 2007 maj;25(5):576-83. https://doi.org/10.1038/nbt1300

Author

Brunner, Erich ; Ahrens, Christian H ; Mohanty, Sonali ; Baetschmann, Hansruedi ; Loevenich, Sandra ; Potthast, Frank ; Deutsch, Eric W ; Panse, Christian ; de Lichtenberg, Ulrik ; Rinner, Oliver ; Lee, Hookeun ; Pedrioli, Patrick G A ; Malmstrom, Johan ; Koehler, Katja ; Schrimpf, Sabine ; Krijgsveld, Jeroen ; Kregenow, Floyd ; Heck, Albert J R ; Hafen, Ernst ; Schlapbach, Ralph ; Aebersold, Ruedi. / A high-quality catalog of the Drosophila melanogaster proteome. I: Nature Biotechnology. 2007 ; Vol. 25, Nr. 5. s. 576-83.

RIS

TY - JOUR

T1 - A high-quality catalog of the Drosophila melanogaster proteome

AU - Brunner, Erich

AU - Ahrens, Christian H

AU - Mohanty, Sonali

AU - Baetschmann, Hansruedi

AU - Loevenich, Sandra

AU - Potthast, Frank

AU - Deutsch, Eric W

AU - Panse, Christian

AU - de Lichtenberg, Ulrik

AU - Rinner, Oliver

AU - Lee, Hookeun

AU - Pedrioli, Patrick G A

AU - Malmstrom, Johan

AU - Koehler, Katja

AU - Schrimpf, Sabine

AU - Krijgsveld, Jeroen

AU - Kregenow, Floyd

AU - Heck, Albert J R

AU - Hafen, Ernst

AU - Schlapbach, Ralph

AU - Aebersold, Ruedi

PY - 2007/5

Y1 - 2007/5

N2 - Understanding how proteins and their complex interaction networks convert the genomic information into a dynamic living organism is a fundamental challenge in biological sciences. As an important step towards understanding the systems biology of a complex eukaryote, we cataloged 63% of the predicted Drosophila melanogaster proteome by detecting 9,124 proteins from 498,000 redundant and 72,281 distinct peptide identifications. This unprecedented high proteome coverage for a complex eukaryote was achieved by combining sample diversity, multidimensional biochemical fractionation and analysis-driven experimentation feedback loops, whereby data collection is guided by statistical analysis of prior data. We show that high-quality proteomics data provide crucial information to amend genome annotation and to confirm many predicted gene models. We also present experimentally identified proteotypic peptides matching approximately 50% of D. melanogaster gene models. This library of proteotypic peptides should enable fast, targeted and quantitative proteomic studies to elucidate the systems biology of this model organism.

AB - Understanding how proteins and their complex interaction networks convert the genomic information into a dynamic living organism is a fundamental challenge in biological sciences. As an important step towards understanding the systems biology of a complex eukaryote, we cataloged 63% of the predicted Drosophila melanogaster proteome by detecting 9,124 proteins from 498,000 redundant and 72,281 distinct peptide identifications. This unprecedented high proteome coverage for a complex eukaryote was achieved by combining sample diversity, multidimensional biochemical fractionation and analysis-driven experimentation feedback loops, whereby data collection is guided by statistical analysis of prior data. We show that high-quality proteomics data provide crucial information to amend genome annotation and to confirm many predicted gene models. We also present experimentally identified proteotypic peptides matching approximately 50% of D. melanogaster gene models. This library of proteotypic peptides should enable fast, targeted and quantitative proteomic studies to elucidate the systems biology of this model organism.

KW - Amino Acid Sequence

KW - Animals

KW - Databases, Protein

KW - Drosophila Proteins

KW - Drosophila melanogaster

KW - Molecular Sequence Data

KW - Proteome

KW - Journal Article

KW - Research Support, N.I.H., Extramural

KW - Research Support, Non-U.S. Gov't

U2 - 10.1038/nbt1300

DO - 10.1038/nbt1300

M3 - Article

VL - 25

SP - 576

EP - 583

JO - Nature Biotechnology

T2 - Nature Biotechnology

JF - Nature Biotechnology

SN - 1546-1696

IS - 5

ER -