A method of predicting the in vitro fibril formation propensity of Aβ40 mutants based on their inclusion body levels in E. coli

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A method of predicting the in vitro fibril formation propensity of Aβ40 mutants based on their inclusion body levels in E. coli. / Sanagavarapu, Kalyani; Nüske, Elisabeth; Nasir, Irem; Meisl, Georg; Immink, Jasper N.; Sormanni, Pietro; Vendruscolo, Michele; Knowles, Tuomas P.J.; Malmendal, Anders; Cabaleiro-Lago, Celia; Linse, Sara.

I: Scientific Reports, Vol. 9, Nr. 1, 3680, 06.03.2019.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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Sanagavarapu, Kalyani ; Nüske, Elisabeth ; Nasir, Irem ; Meisl, Georg ; Immink, Jasper N. ; Sormanni, Pietro ; Vendruscolo, Michele ; Knowles, Tuomas P.J. ; Malmendal, Anders ; Cabaleiro-Lago, Celia ; Linse, Sara. / A method of predicting the in vitro fibril formation propensity of Aβ40 mutants based on their inclusion body levels in E. coli. I: Scientific Reports. 2019 ; Vol. 9, Nr. 1.

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TY - JOUR

T1 - A method of predicting the in vitro fibril formation propensity of Aβ40 mutants based on their inclusion body levels in E. coli

AU - Sanagavarapu, Kalyani

AU - Nüske, Elisabeth

AU - Nasir, Irem

AU - Meisl, Georg

AU - Immink, Jasper N.

AU - Sormanni, Pietro

AU - Vendruscolo, Michele

AU - Knowles, Tuomas P.J.

AU - Malmendal, Anders

AU - Cabaleiro-Lago, Celia

AU - Linse, Sara

PY - 2019/3/6

Y1 - 2019/3/6

N2 - Overexpression of recombinant proteins in bacteria may lead to their aggregation and deposition in inclusion bodies. Since the conformational properties of proteins in inclusion bodies exhibit many of the characteristics typical of amyloid fibrils. Based on these findings, we hypothesize that the rate at which proteins form amyloid fibrils may be predicted from their propensity to form inclusion bodies. To establish a method based on this concept, we first measured by SDS-PAGE and confocal microscopy the level of inclusion bodies in E. coli cells overexpressing the 40-residue amyloid-beta peptide, Aβ40, wild-type and 24 charge mutants. We then compared these results with a number of existing computational aggregation propensity predictors as well as the rates of aggregation measured in vitro for selected mutants. Our results show a strong correlation between the level of inclusion body formation and aggregation propensity, thus demonstrating the power of this approach and its value in identifying factors modulating aggregation kinetics.

AB - Overexpression of recombinant proteins in bacteria may lead to their aggregation and deposition in inclusion bodies. Since the conformational properties of proteins in inclusion bodies exhibit many of the characteristics typical of amyloid fibrils. Based on these findings, we hypothesize that the rate at which proteins form amyloid fibrils may be predicted from their propensity to form inclusion bodies. To establish a method based on this concept, we first measured by SDS-PAGE and confocal microscopy the level of inclusion bodies in E. coli cells overexpressing the 40-residue amyloid-beta peptide, Aβ40, wild-type and 24 charge mutants. We then compared these results with a number of existing computational aggregation propensity predictors as well as the rates of aggregation measured in vitro for selected mutants. Our results show a strong correlation between the level of inclusion body formation and aggregation propensity, thus demonstrating the power of this approach and its value in identifying factors modulating aggregation kinetics.

U2 - 10.1038/s41598-019-39216-z

DO - 10.1038/s41598-019-39216-z

M3 - Article

VL - 9

JO - Scientific Reports

T2 - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 3680

ER -