A Microfluidic Platform for Real-Time Detection and Quantification of Protein-Ligand Interactions

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

The key steps in cellular signaling and regulatory pathways rely on reversible noncovalent protein-ligand binding, yet the equilibrium parameters for such events remain challenging to characterize and quantify in solution. Here, we demonstrate a microfluidic platform for the detection of protein-ligand interactions with an assay time on the second timescale and without the requirement for immobilization or the presence of a highly viscous matrix. Using this approach, we obtain absolute values for the electrophoretic mobilities characterizing solvated proteins and demonstrate quantitative comparison of results obtained under different solution conditions. We apply this strategy to characterize the interaction between calmodulin and creatine kinase, which we identify as a novel calmodulin target. Moreover, we explore the differential calcium ion dependence of calmodulin ligand-binding affinities, a system at the focal point of calcium-mediated cellular signaling pathways. We further explore the effect of calmodulin on creatine kinase activity and show that it is increased by the interaction between the two proteins. These findings demonstrate the potential of quantitative microfluidic techniques to characterize binding equilibria between biomolecules under native solution conditions.

Detaljer

Författare
  • Therese W. Herling
  • David J. O'Connell
  • Mikael C. Bauer
  • Jonas Persson
  • Ulrich Weininger
  • Tuomas P J Knowles
  • Sara Linse
Enheter & grupper
Externa organisationer
  • University of Cambridge
  • University College Dublin
  • Uppsala universitet
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biofysik
Originalspråkengelska
Sidor (från-till)1957-1966
Antal sidor10
TidskriftBiophysical Journal
Volym110
Utgåva nummer9
StatusPublished - 2016 maj 10
PublikationskategoriForskning
Peer review utfördJa