A new standardized lipolysis approach for characterization of emulsions and dispersions

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Standard

A new standardized lipolysis approach for characterization of emulsions and dispersions. / Brogard, M.; Troedsson, E; Thuresson, Krister; Ljusberg, Helena.

I: Journal of Colloid and Interface Science, Vol. 308, Nr. 2, 2007, s. 500-507.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

APA

CBE

MLA

Vancouver

Author

Brogard, M. ; Troedsson, E ; Thuresson, Krister ; Ljusberg, Helena. / A new standardized lipolysis approach for characterization of emulsions and dispersions. I: Journal of Colloid and Interface Science. 2007 ; Vol. 308, Nr. 2. s. 500-507.

RIS

TY - JOUR

T1 - A new standardized lipolysis approach for characterization of emulsions and dispersions

AU - Brogard, M.

AU - Troedsson, E

AU - Thuresson, Krister

AU - Ljusberg, Helena

PY - 2007

Y1 - 2007

N2 - A new standardized lipolysis approach is presented where the focus is on the initial rate of lipolysis. An advantage is that data obtained in this way reflect degradation before growing amounts of lipolysis products retard the process. The method can be used to rank different lipase substrates. In particular, the method can be used to obtain information about the susceptibility to degradation of various emulsions and dispersions that are used in technical applications. We present how the method is standardized to facilitate comparison of various substrates. This involves (i) lipase substrate in excess, i.e., the amount of lipase is rate limiting, and (ii) expressing rate of degradation relative to that of a reference substrate, tributyrin. Under such conditions, with the amount of lipase substrate held constant, an increase in enzymatic activity will generate a proportional increase in the lipolysis rate. This enables comparison of results obtained from different enzyme batches and corrects for day-to-day variability. Examples illustrating the potential of the method to discriminate and rank different lipase substrates with regard to enzymatic degradation are presented. (c) 2006 Elsevier Inc. All rights reserved.

AB - A new standardized lipolysis approach is presented where the focus is on the initial rate of lipolysis. An advantage is that data obtained in this way reflect degradation before growing amounts of lipolysis products retard the process. The method can be used to rank different lipase substrates. In particular, the method can be used to obtain information about the susceptibility to degradation of various emulsions and dispersions that are used in technical applications. We present how the method is standardized to facilitate comparison of various substrates. This involves (i) lipase substrate in excess, i.e., the amount of lipase is rate limiting, and (ii) expressing rate of degradation relative to that of a reference substrate, tributyrin. Under such conditions, with the amount of lipase substrate held constant, an increase in enzymatic activity will generate a proportional increase in the lipolysis rate. This enables comparison of results obtained from different enzyme batches and corrects for day-to-day variability. Examples illustrating the potential of the method to discriminate and rank different lipase substrates with regard to enzymatic degradation are presented. (c) 2006 Elsevier Inc. All rights reserved.

KW - self-emulsifying drug delivery systems (SEDDS)

KW - lipolysis

KW - digestion

KW - lipid

KW - lipid-based drug delivery

KW - structured lipids

KW - lamellar

KW - dispersion

KW - triglyceride

KW - emulsion

U2 - 10.1016/j.jcis.2006.12.015

DO - 10.1016/j.jcis.2006.12.015

M3 - Article

VL - 308

SP - 500

EP - 507

JO - Journal of Colloid and Interface Science

T2 - Journal of Colloid and Interface Science

JF - Journal of Colloid and Interface Science

SN - 0021-9797

IS - 2

ER -