A novel proteomic approach reveals a role for Mg-protoporphyrin IX in response to oxidative stress

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Bibtex

@article{ccfc65666cbe408798afa3264d8db521,
title = "A novel proteomic approach reveals a role for Mg-protoporphyrin IX in response to oxidative stress",
abstract = "The presence of genes encoding organellar proteins in different cellular compartments necessitates a tight coordination of expression by the different genomes of the eukaryotic cell. This coordination of gene expression is achieved by organelle-to-nucleus communication. Stress-induced perturbations of the tetrapyrrole pathway trigger large changes in nuclear gene expression. In order to investigate whether the tetrapyrrole Mg-ProtoIX itself is an important part of plastid-to-nucleus communication, we used an affinity column containing Mg-ProtoIX covalently linked to an Affi-Gel matrix. The proteins that bound to Mg-ProtoIX were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis combined with nano liquid chromatography-mass spectrometry (MS)/MS. Thus, we present a novel proteomic approach to address the mechanisms involved in cellular signaling and we identified interactions between Mg-ProtoIX and a large number of proteins associated with oxidative stress responses. Our approach revealed an interaction between Mg-ProtoIX and the heat shock protein 90-type protein, HSP81-2 suggesting that a regulatory complex including HSP90 proteins and tetrapyrroles controlling gene expression is evolutionarily conserved between yeast and plants. In addition, our list of putative Mg-ProtoIX-binding proteins demonstrated that binding of tetrapyrroles does not depend on a specific amino acid motif but possibly on a specific fold of the protein.",
keywords = "Protoporphyrins/*metabolism, Proteomics/*methods, Protein Subunits/metabolism, Protein Binding, *Oxidative Stress, Lyases/metabolism, Light-Harvesting Protein Complexes/metabolism, Plant, Gene Expression Regulation, Computational Biology, Western, Blotting, Arabidopsis Proteins/genetics/metabolism, Amino Acid Motifs, Arabidopsis/enzymology/genetics, Reproducibility of Results, Signal Transduction, Spectrometry, Fluorescence, Stress, Physiological, Tetrapyrroles/metabolism",
author = "P. Kindgren and Eriksson, {M. J.} and C. Benedict and A. Mohapatra and Gough, {S. P.} and Mats Hansson and T. Kieselbach and A. Strand",
note = "4",
year = "2011",
doi = "10.1111/j.1399-3054.2010.01440.x",
language = "English",
volume = "141",
pages = "310--320",
journal = "Physiologia Plantarum",
issn = "0031-9317",
publisher = "John Wiley and Sons",
number = "4",

}