A novel, robust method for quantification of multiple kynurenine pathway metabolites in the cerebrospinal fluid

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Abstract

Aim: Kynurenine metabolites are potential modulators of psychiatric disease. We aimed to develop a highly sensitive biochemical analysis of cerebrospinal fluid (CSF) tryptophan (TRP) metabolites, to investigate the stability of metabolites and to confirm our previous findings of aberrant CSF quinolinic acid (QUIN) and picolinic acid (PIC) in suicide attempters using this method. Methodology & results: Ten CSF TRP metabolites were analyzed with ultraperformance LC-MS/MS. The method showed small intra- and interassay variation. Metabolites were stable following freeze-thaw cycles. A decreased CSF PIC/QUIN ratio was found in suicide attempters. Conclusion: The feasibility of reliably determining CSF TRP metabolites were demonstrated, including separation of the two isomers PIC and nicotinic acid (NA) and the finding of a reduced PIC/QUIN ratio replicated in suicide attempters.

Detaljer

Författare
  • Lilly Schwieler
  • Ada Trepci
  • Stanislaw Krzyzanowski
  • Sigurd Hermansson
  • Mathias Granqvist
  • Fredrik Piehl
  • Tomas Venckunas
  • Marius Brazaitis
  • Sigitas Kamandulis
  • Daniel Lindqvist
  • Arthur Daniel Jones
  • Sophie Erhardt
  • Lena Brundin
Enheter & grupper
Externa organisationer
  • Karolinska Institute
  • Van Andel Research Institute
  • Karolinska University Hospital
  • Michigan State University
  • Waters Sweden AB
  • Lithuanian Sports University
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Neurologi
  • Analytisk kemi
  • Psykiatri

Nyckelord

Originalspråkengelska
Sidor (från-till)379-392
Antal sidor14
TidskriftBioanalysis
Volym12
Utgåva nummer6
StatusPublished - 2020 mar 25
PublikationskategoriForskning
Peer review utfördJa