A porous silicon immunoassay platform for fluorometric determination of alpha-synuclein in human cerebrospinal fluid

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Levels of total and/or oligomeric alpha-synuclein may be used as a biomarker tool to aid in the diagnosis and development of new disease-modifying therapies. We report here on a porous silicon antibody microarray for the fluorimetric determination of cerebrospinal fluid levels of total alpha-synuclein, a protein involved the pathology of Parkinson's disease. The surface of porous silicon has a 3-dimensional macro- and nanoporous structure, and this offers a large binding capacity for capturing probe molecules. Porous silicon also warrants efficient immobilization of antibodies by surface adsorption, and does not require chemical immobilization. The platform requires 10 mu L of cerebrospinal fluid, and each test requires 4 h for assay only (including immobilization of capturing antibody). The limit of detection is 35 pg mL(-1) of alpha-synuclein in cerebrospinal fluid, and the dynamic analytical range extends from 0.01 to 100 ng center dot mL(-1).

Detaljer

Författare
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Neurovetenskaper
  • Neurologi

Nyckelord

Originalspråkengelska
Sidor (från-till)1143-1149
TidskriftMikrochimica Acta
Volym181
Utgåva nummer9-10
StatusPublished - 2014
PublikationskategoriForskning
Peer review utfördJa