A simple and fast liquid-liquid extraction method for the determination of 2,2 ',4,4 ',5,5 '-hexachlorobiphenyl (CB-153) and 1,1-dichloro-2,2-bis(p-chlorophenyl)-ethylene (p,p '-DDE) from human serum for epidemiological studies on type 2 diabetes

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Bibtex

@article{5abc76a7e3714202945e9393908ba806,
title = "A simple and fast liquid-liquid extraction method for the determination of 2,2 ',4,4 ',5,5 '-hexachlorobiphenyl (CB-153) and 1,1-dichloro-2,2-bis(p-chlorophenyl)-ethylene (p,p '-DDE) from human serum for epidemiological studies on type 2 diabetes",
abstract = "A simple and fast method is presented to be used for example in studies on the relationship between serum levels of persistent organic pollutants and type 2 diabetes mellitus. Method is based on liquid-liquid extraction and gas chromatography coupled with high.-resolution mass spectrometry. In the sample pretreatment special attention was paid to minimize the number of sample manipulation steps and the amounts of organic solvents needed. Compounds analyzed were 2,2',4,4',5,5'-hexachlorobiphenyl (PCB-153) and 1,1-dichloro-2,2-bis (p-chlorophenyl)-ethylene (p,p'-DDE), the major metabolite of DDT. The method included extraction and cleanup of 0.2 rill of serum in a single test tube and subsequent analysis of the extract from 0.2 ml final volume. Validation was conducted to explore the performance of the method. The limits of detection for p,p'-DDE and PCB-153 based on the standard deviation of the blank samples were 4.3 and 3.1 pg/ml, respectively. Repeatability was less than 2.5{\%} at three concentration levels tested and recovery from Certified Reference Material SRM 1589a was 84{\%} for p,p'-DDE and 87{\%} for PCB-153 of the certified values, respectively. Serum samples from the AMAP intercalibration round 2008-2 were also analyzed, and results were 101-116{\%} of the assigned values. The presented method was used for an epidemiological study with more than 700 serum samples from a type 2 diabetes cohort from Sweden. (C) 2008 Elsevier B.V. All rights reserved.",
keywords = "PCB-153, p, p '-DDE, organic pollutants (POPs), Persistent, Type 2 diabetes, Liquid-liquid extraction, Human serum",
author = "Panu Rantakokko and Hannu Kiviranta and Lars Rylander and Anna Rignell-Hydbom and Terttu Vartiainen",
year = "2009",
doi = "10.1016/j.chroma.2008.12.013",
language = "English",
volume = "1216",
pages = "897--901",
journal = "Journal of Chromatography",
issn = "0021-9673",
publisher = "Elsevier",
number = "6",

}