A simple assay for the detection of antibodies to endocrine islet cell surface antigens

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

A simple and sensitive immunoradiometric assay for the detection of islet cell surface antibodies (CIRMA) has been developed. Live, transformed islet cells derived from a liver metastasis of a transplantable islet cell tumor were grown in removable microtiter wells and incubated with antibody. Cell-bound antibodies were quantitated using 125I-labelled second antibodies. The assay was used to detect islet cell antibodies present in sera from non-diabetic and diabetic BB rats and proved to be particularly effective for screening hybridoma supernatants in order to identify monoclonal antibodies recognizing islet cell surface antigens.

Detaljer

Författare
Externa organisationer
  • Hagedorn Research Institute
Forskningsområden

Nyckelord

Originalspråkengelska
Sidor (från-till)135-139
Antal sidor5
TidskriftJournal of Immunological Methods
Volym95
Utgåva nummer1
StatusPublished - 1986 dec 4
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa