A simple protocol to obtain highly pure Wolbachia endosymbiont DNA for genome sequencing

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Most genome sequencing projects using intracellular bacteria face difficulties in obtaining sufficient bacterial DNA free of host contamination. We have developed a simple and rapid protocol to isolate endosymbiont DNA virtually free from fly and mosquito host DNA. We purified DNA from six Wolbachia strains in preparation for genome sequencing using this method, and achieved up to 97% pure Wolbachia sequence, even after using frozen insects. This is a significant improvement for future Wolbachia and other endosymbiont genome projects.

Detaljer

Författare
  • Inaki Iturbe-Ormaetxe
  • Megan Woolfit
  • Edwige Rances
  • Anne Duplouy
  • Scott L. O'NEill
Externa organisationer
  • University of Queensland
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Genetik
Originalspråkengelska
Sidor (från-till)134-136
TidskriftJournal of Microbiological Methods
Volym84
Utgivningsnummer1
StatusPublished - 2011
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa