A soft tick Ornithodoros moubata salivary protein OmCI is a potent inhibitor to prevent avian complement activation

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A soft tick Ornithodoros moubata salivary protein OmCI is a potent inhibitor to prevent avian complement activation. / Frye, Amber M.; Hart, Thomas M.; Tufts, Danielle M.; Ram, Sanjay; Diuk-Wasser, Maria A.; Kraiczy, Peter; Blom, Anna M.; Lin, Yi Pin.

I: Ticks and Tick-borne Diseases, Vol. 11, Nr. 2, 101354, 03.2020.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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Frye, A. M., Hart, T. M., Tufts, D. M., Ram, S., Diuk-Wasser, M. A., Kraiczy, P., ... Lin, Y. P. (2020). A soft tick Ornithodoros moubata salivary protein OmCI is a potent inhibitor to prevent avian complement activation. Ticks and Tick-borne Diseases, 11(2), [101354]. https://doi.org/10.1016/j.ttbdis.2019.101354

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Frye, Amber M. ; Hart, Thomas M. ; Tufts, Danielle M. ; Ram, Sanjay ; Diuk-Wasser, Maria A. ; Kraiczy, Peter ; Blom, Anna M. ; Lin, Yi Pin. / A soft tick Ornithodoros moubata salivary protein OmCI is a potent inhibitor to prevent avian complement activation. I: Ticks and Tick-borne Diseases. 2020 ; Vol. 11, Nr. 2.

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TY - JOUR

T1 - A soft tick Ornithodoros moubata salivary protein OmCI is a potent inhibitor to prevent avian complement activation

AU - Frye, Amber M.

AU - Hart, Thomas M.

AU - Tufts, Danielle M.

AU - Ram, Sanjay

AU - Diuk-Wasser, Maria A.

AU - Kraiczy, Peter

AU - Blom, Anna M.

AU - Lin, Yi Pin

PY - 2020/3

Y1 - 2020/3

N2 - Complement is a key first line innate host defense system in the blood of vertebrates. Upon activation, this powerful defense mechanism can elicit inflammatory responses, lyse non-self-cells, or mark them for opsonophagocytic removal. Blood-feeding arthropods thus require the ability to block host complement activation in the bloodmeal to prevent undesired cell or tissue damage during feeding. The soft tick Ornithodoros moubata produces a complement inhibitory protein, OmCI. This protein binds to a mammalian complement protein C5 and blocks further activation of complement cascades, which results in the prevention of complement-mediated bacterial killing through membrane attack complex. Interestingly, the amino acids involved in OmCI binding are highly conserved among mammalian and avian C5, but the ability of this protein to inhibit the complement from birds remains unclear. Here we demonstrated that OmCI is capable of preventing quail complement-mediated erythrocyte lysis, inhibiting the capability of this animal's complement to eliminate a serum-sensitive Lyme disease bacterial strain. We also found that the ability of OmCI to inhibit quail complement-mediated killing of Lyme disease bacteria can be extended to different domestic and wild birds. Our results illustrate the utility of OmCI to block bird complement. These results provide the foundation for further use of this protein as a tool to study the molecular basis of avian complement and pathogen evasion to such a defense mechanism.

AB - Complement is a key first line innate host defense system in the blood of vertebrates. Upon activation, this powerful defense mechanism can elicit inflammatory responses, lyse non-self-cells, or mark them for opsonophagocytic removal. Blood-feeding arthropods thus require the ability to block host complement activation in the bloodmeal to prevent undesired cell or tissue damage during feeding. The soft tick Ornithodoros moubata produces a complement inhibitory protein, OmCI. This protein binds to a mammalian complement protein C5 and blocks further activation of complement cascades, which results in the prevention of complement-mediated bacterial killing through membrane attack complex. Interestingly, the amino acids involved in OmCI binding are highly conserved among mammalian and avian C5, but the ability of this protein to inhibit the complement from birds remains unclear. Here we demonstrated that OmCI is capable of preventing quail complement-mediated erythrocyte lysis, inhibiting the capability of this animal's complement to eliminate a serum-sensitive Lyme disease bacterial strain. We also found that the ability of OmCI to inhibit quail complement-mediated killing of Lyme disease bacteria can be extended to different domestic and wild birds. Our results illustrate the utility of OmCI to block bird complement. These results provide the foundation for further use of this protein as a tool to study the molecular basis of avian complement and pathogen evasion to such a defense mechanism.

KW - Avian complement

KW - Bacterial killing

KW - Lyme borreliae

KW - OmCI

U2 - 10.1016/j.ttbdis.2019.101354

DO - 10.1016/j.ttbdis.2019.101354

M3 - Article

VL - 11

JO - Ticks and Tick-borne Diseases

JF - Ticks and Tick-borne Diseases

SN - 1877-9603

IS - 2

M1 - 101354

ER -