A sol-gel integrated dual-readout microarray platform for quantification and identification of prostate-specific antigen

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Here, we report a sol-gel integrated affinity microarray for on-chip matrix-assisted laser desorption/ionization time-offlight mass spectrometry (MALDI-TOF-MS) that enables capture and identification of prostate-specific antigen (PSA) in samples. An anti-PSA antibody (H117) was mixed with a sol-gel, and the mixture was spotted onto a porous silicon (pSi) surface without additional surface modifications. The antibody easily penetrates the sol-gel macropore fluidic network structure, making possible high affinities. To assess the capture affinity of the platform, we performed a direct assay using fluorescein isothiocyanate-labeled PSA. Pure PSA was subjected to on-chip MALDI-TOF-MS analysis, yielding three clear mass peptide peaks (m/z = 1272, 1407, and 1872). The sol-gel microarray platform enables dual readout of PSA both fluorometric and MALDI-TOF MS analysis in biological samples. Here we report a useful method for a means for discovery of biomarkers in complex body fluids.

Detaljer

Författare
  • Sang Wook Lee
  • Jong Hyun Lee
  • Hyuck Gi Kwon
  • Thomas Laurell
  • Ok Chan Jeong
  • Soyoun Kim
Enheter & grupper
Externa organisationer
  • University of Tokyo
  • Dongguk University, Seoul
  • Inje University
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Medicinsk laboratorie- och mätteknik

Nyckelord

Originalspråkengelska
Sidor (från-till)317-321
Antal sidor5
TidskriftAnalytical Sciences
Volym34
Utgåva nummer3
StatusPublished - 2018
PublikationskategoriForskning
Peer review utfördJa