Absence of Parallel Fibre to Purkinje Cell LTD During Eyeblink Conditioning

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Absence of Parallel Fibre to Purkinje Cell LTD During Eyeblink Conditioning. / Johansson, Fredrik; Jirenhed, Dan Anders; Rasmussen, Anders; Zucca, Riccardo; Hesslow, Germund.

I: Scientific Reports, Vol. 8, Nr. 1, 14777, 01.12.2018.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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TY - JOUR

T1 - Absence of Parallel Fibre to Purkinje Cell LTD During Eyeblink Conditioning

AU - Johansson, Fredrik

AU - Jirenhed, Dan Anders

AU - Rasmussen, Anders

AU - Zucca, Riccardo

AU - Hesslow, Germund

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Long-term depression (LTD) of parallel fibre/Purkinje cell synapses has been the favoured explanation for cerebellar motor learning such as classical eyeblink conditioning. Previous evidence against this interpretation has been contested. Here we wanted to test whether a classical conditioning protocol causes LTD. We applied a conditioning protocol, using a train of electrical pulses to the parallel fibres as the conditional stimulus. In order to rule out indirect effects caused by antidromic granule cell activation or output from Purkinje cells that might produce changes in Purkinje cell responsiveness, we focused the analysis on the first pulse in the conditional stimulus, that is, before any indirect effects would have time to occur. Purkinje cells learned to respond with a firing pause to the conditional stimulus. Yet, there was no depression of parallel fibre excitation after training.

AB - Long-term depression (LTD) of parallel fibre/Purkinje cell synapses has been the favoured explanation for cerebellar motor learning such as classical eyeblink conditioning. Previous evidence against this interpretation has been contested. Here we wanted to test whether a classical conditioning protocol causes LTD. We applied a conditioning protocol, using a train of electrical pulses to the parallel fibres as the conditional stimulus. In order to rule out indirect effects caused by antidromic granule cell activation or output from Purkinje cells that might produce changes in Purkinje cell responsiveness, we focused the analysis on the first pulse in the conditional stimulus, that is, before any indirect effects would have time to occur. Purkinje cells learned to respond with a firing pause to the conditional stimulus. Yet, there was no depression of parallel fibre excitation after training.

U2 - 10.1038/s41598-018-32791-7

DO - 10.1038/s41598-018-32791-7

M3 - Article

VL - 8

JO - Scientific Reports

T2 - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 14777

ER -