Acoustic actuated fluorescence activated sorting of microparticles.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

In this paper, we present a fluorescence activated sorter realized in a continuous flow microfluidic chip. Sorting is achieved by deflecting a focused particle stream with short acoustic bursts (2.5 ms), in a fluorescence activated configuration. The system utilizes two-dimensional acoustic pre-focusing, using a single actuation frequency, to position all particles in the same fluid velocity regime at flow rates up to 1.7 mL min(-1). Particles were sorted based on their fluorescence intensities at throughputs up to 150 particles s(-1). The highest purity reached was 80% when sorting at an average rate of 50 particles s(-1). The average recovery of a sort was 93.2 ± 2.6%. The presented system enables fluorescence activated cell sorting in a continuous flow microfluidic format that allows aseptic integration of downstream microfluidic functionalities, opening for medical and clinical applications.

Detaljer

Författare
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Medicinsk laboratorie- och mätteknik
Originalspråkengelska
Sidor (från-till)1943-1950
TidskriftLab on a Chip
Volym14
Utgivningsnummer11
StatusPublished - 2014
PublikationskategoriForskning
Peer review utfördJa

Relaterad forskningsoutput

Jakobsson, O., 2016 okt 17, Lund: Department of Biomedical Engineering, Lund university. 123 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

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