Actomyosin motility on nanostructured surfaces

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Bibtex

@article{85b4439ce610428ea3108667b954b428,
title = "Actomyosin motility on nanostructured surfaces",
abstract = "We have here, for the first time, used nanofabrication techniques to reproduce aspects of the ordered actomyosin arrangement in a muscle cell. The adsorption of functional heavy meromyosin (HMM) to five different resist polymers was first assessed. One group of resists (MRL-6000.1XP and ZEP-520) consistently exhibited high quality motility of actin filaments after incubation with HMM. A second group (PMMA-200, PMMA-950, and MRI-9030) generally gave low quality of motility with only few smoothly moving filaments. Based on these findings electron beam lithography was applied to a bi-layer resist system with PMMA-950 on top of MRL-6000.1XP. Grooves (100-200 nm wide) in the PMMA layer were created to expose the MRL-6000.1XP surface for adsorption of HMM and guidance of actin filament motility. This guidance was quite efficient allowing no U-turns of the filaments and approximately 20 times higher density of moving filaments in the grooves than on the surrounding PMMA.",
keywords = "beam lithography, electron, nanotechnology, resist polymer, motility assay, actin, myosin, atomic force microscope",
author = "Richard Bunk and J Klinth and Lars Montelius and IA Nicholls and P{\"a}r Omling and S Tagerud and A Mansson",
year = "2003",
doi = "10.1016/S0006-291X(03)00027-5",
language = "English",
volume = "301",
pages = "783--788",
journal = "Biochemical and Biophysical Research Communications",
issn = "1090-2104",
publisher = "Elsevier",
number = "3",

}