Actomyosin motility on nanostructured surfaces

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Standard

Actomyosin motility on nanostructured surfaces. / Bunk, Richard; Klinth, J; Montelius, Lars; Nicholls, IA; Omling, Pär; Tagerud, S; Mansson, A.

I: Biochemical and Biophysical Research Communications, Vol. 301, Nr. 3, 2003, s. 783-788.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

APA

CBE

MLA

Vancouver

Author

Bunk, Richard ; Klinth, J ; Montelius, Lars ; Nicholls, IA ; Omling, Pär ; Tagerud, S ; Mansson, A. / Actomyosin motility on nanostructured surfaces. I: Biochemical and Biophysical Research Communications. 2003 ; Vol. 301, Nr. 3. s. 783-788.

RIS

TY - JOUR

T1 - Actomyosin motility on nanostructured surfaces

AU - Bunk, Richard

AU - Klinth, J

AU - Montelius, Lars

AU - Nicholls, IA

AU - Omling, Pär

AU - Tagerud, S

AU - Mansson, A

PY - 2003

Y1 - 2003

N2 - We have here, for the first time, used nanofabrication techniques to reproduce aspects of the ordered actomyosin arrangement in a muscle cell. The adsorption of functional heavy meromyosin (HMM) to five different resist polymers was first assessed. One group of resists (MRL-6000.1XP and ZEP-520) consistently exhibited high quality motility of actin filaments after incubation with HMM. A second group (PMMA-200, PMMA-950, and MRI-9030) generally gave low quality of motility with only few smoothly moving filaments. Based on these findings electron beam lithography was applied to a bi-layer resist system with PMMA-950 on top of MRL-6000.1XP. Grooves (100-200 nm wide) in the PMMA layer were created to expose the MRL-6000.1XP surface for adsorption of HMM and guidance of actin filament motility. This guidance was quite efficient allowing no U-turns of the filaments and approximately 20 times higher density of moving filaments in the grooves than on the surrounding PMMA.

AB - We have here, for the first time, used nanofabrication techniques to reproduce aspects of the ordered actomyosin arrangement in a muscle cell. The adsorption of functional heavy meromyosin (HMM) to five different resist polymers was first assessed. One group of resists (MRL-6000.1XP and ZEP-520) consistently exhibited high quality motility of actin filaments after incubation with HMM. A second group (PMMA-200, PMMA-950, and MRI-9030) generally gave low quality of motility with only few smoothly moving filaments. Based on these findings electron beam lithography was applied to a bi-layer resist system with PMMA-950 on top of MRL-6000.1XP. Grooves (100-200 nm wide) in the PMMA layer were created to expose the MRL-6000.1XP surface for adsorption of HMM and guidance of actin filament motility. This guidance was quite efficient allowing no U-turns of the filaments and approximately 20 times higher density of moving filaments in the grooves than on the surrounding PMMA.

KW - beam lithography

KW - electron

KW - nanotechnology

KW - resist polymer

KW - motility assay

KW - actin

KW - myosin

KW - atomic force microscope

U2 - 10.1016/S0006-291X(03)00027-5

DO - 10.1016/S0006-291X(03)00027-5

M3 - Article

VL - 301

SP - 783

EP - 788

JO - Biochemical and Biophysical Research Communications

T2 - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 1090-2104

IS - 3

ER -