Alcohol dehydrogenase gene ADH3 activates glucose alcoholic fermentation in genetically engineered Dekkera bruxellensis yeast.

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Alcohol dehydrogenase gene ADH3 activates glucose alcoholic fermentation in genetically engineered Dekkera bruxellensis yeast. / Schifferdecker, Anna; Siurkas, Juozas; Andersen, Mikael Rørdam; Joerck-Ramberg, Dorte; Ling, Zhihao; Zhou, Nerve; Blevins, James E; Sibirny, Andriy A; Piskur, Jure; Ishchuk, Olena.

I: Applied Microbiology and Biotechnology, 2016.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Schifferdecker, A, Siurkas, J, Andersen, MR, Joerck-Ramberg, D, Ling, Z, Zhou, N, Blevins, JE, Sibirny, AA, Piskur, J & Ishchuk, O 2016, 'Alcohol dehydrogenase gene ADH3 activates glucose alcoholic fermentation in genetically engineered Dekkera bruxellensis yeast.', Applied Microbiology and Biotechnology. https://doi.org/10.1007/s00253-015-7266-x

APA

Schifferdecker, A., Siurkas, J., Andersen, M. R., Joerck-Ramberg, D., Ling, Z., Zhou, N., Blevins, J. E., Sibirny, A. A., Piskur, J., & Ishchuk, O. (2016). Alcohol dehydrogenase gene ADH3 activates glucose alcoholic fermentation in genetically engineered Dekkera bruxellensis yeast. Applied Microbiology and Biotechnology. https://doi.org/10.1007/s00253-015-7266-x

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Author

Schifferdecker, Anna ; Siurkas, Juozas ; Andersen, Mikael Rørdam ; Joerck-Ramberg, Dorte ; Ling, Zhihao ; Zhou, Nerve ; Blevins, James E ; Sibirny, Andriy A ; Piskur, Jure ; Ishchuk, Olena. / Alcohol dehydrogenase gene ADH3 activates glucose alcoholic fermentation in genetically engineered Dekkera bruxellensis yeast. I: Applied Microbiology and Biotechnology. 2016.

RIS

TY - JOUR

T1 - Alcohol dehydrogenase gene ADH3 activates glucose alcoholic fermentation in genetically engineered Dekkera bruxellensis yeast.

AU - Schifferdecker, Anna

AU - Siurkas, Juozas

AU - Andersen, Mikael Rørdam

AU - Joerck-Ramberg, Dorte

AU - Ling, Zhihao

AU - Zhou, Nerve

AU - Blevins, James E

AU - Sibirny, Andriy A

AU - Piskur, Jure

AU - Ishchuk, Olena

PY - 2016

Y1 - 2016

N2 - Dekkera bruxellensis is a non-conventional Crabtree-positive yeast with a good ethanol production capability. Compared to Saccharomyces cerevisiae, its tolerance to acidic pH and its utilization of alternative carbon sources make it a promising organism for producing biofuel. In this study, we developed an auxotrophic transformation system and an expression vector, which enabled the manipulation of D. bruxellensis, thereby improving its fermentative performance. Its gene ADH3, coding for alcohol dehydrogenase, was cloned and overexpressed under the control of the strong and constitutive promoter TEF1. Our recombinant D. bruxellensis strain displayed 1.4 and 1.7 times faster specific glucose consumption rate during aerobic and anaerobic glucose fermentations, respectively; it yielded 1.2 times and 1.5 times more ethanol than did the parental strain under aerobic and anaerobic conditions, respectively. The overexpression of ADH3 in D. bruxellensis also reduced the inhibition of fermentation by anaerobiosis, the "Custer effect". Thus, the fermentative capacity of D. bruxellensis could be further improved by metabolic engineering.

AB - Dekkera bruxellensis is a non-conventional Crabtree-positive yeast with a good ethanol production capability. Compared to Saccharomyces cerevisiae, its tolerance to acidic pH and its utilization of alternative carbon sources make it a promising organism for producing biofuel. In this study, we developed an auxotrophic transformation system and an expression vector, which enabled the manipulation of D. bruxellensis, thereby improving its fermentative performance. Its gene ADH3, coding for alcohol dehydrogenase, was cloned and overexpressed under the control of the strong and constitutive promoter TEF1. Our recombinant D. bruxellensis strain displayed 1.4 and 1.7 times faster specific glucose consumption rate during aerobic and anaerobic glucose fermentations, respectively; it yielded 1.2 times and 1.5 times more ethanol than did the parental strain under aerobic and anaerobic conditions, respectively. The overexpression of ADH3 in D. bruxellensis also reduced the inhibition of fermentation by anaerobiosis, the "Custer effect". Thus, the fermentative capacity of D. bruxellensis could be further improved by metabolic engineering.

U2 - 10.1007/s00253-015-7266-x

DO - 10.1007/s00253-015-7266-x

M3 - Article

C2 - 26743658

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 1432-0614

ER -