Allergome-wide peptide microarrays enable epitope deconvolution in allergen-specific immunotherapy

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Allergome-wide peptide microarrays enable epitope deconvolution in allergen-specific immunotherapy. / Mikus, Maria; Zandian, Arash; Sjöberg, Ronald; Hamsten, Carl; Forsström, Björn; Andersson, Morgan; Greiff, Lennart; Uhlén, Mathias; Levin, Mattias; Nilsson, Peter; van Hage, Marianne; Ohlin, Mats.

I: Journal of Allergy and Clinical Immunology, Vol. 147, Nr. 3, 2021, s. 1077-1086.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Mikus, M, Zandian, A, Sjöberg, R, Hamsten, C, Forsström, B, Andersson, M, Greiff, L, Uhlén, M, Levin, M, Nilsson, P, van Hage, M & Ohlin, M 2021, 'Allergome-wide peptide microarrays enable epitope deconvolution in allergen-specific immunotherapy', Journal of Allergy and Clinical Immunology, vol. 147, nr. 3, s. 1077-1086. https://doi.org/10.1016/j.jaci.2020.08.002

APA

Mikus, M., Zandian, A., Sjöberg, R., Hamsten, C., Forsström, B., Andersson, M., Greiff, L., Uhlén, M., Levin, M., Nilsson, P., van Hage, M., & Ohlin, M. (2021). Allergome-wide peptide microarrays enable epitope deconvolution in allergen-specific immunotherapy. Journal of Allergy and Clinical Immunology, 147(3), 1077-1086. https://doi.org/10.1016/j.jaci.2020.08.002

CBE

Mikus M, Zandian A, Sjöberg R, Hamsten C, Forsström B, Andersson M, Greiff L, Uhlén M, Levin M, Nilsson P, van Hage M, Ohlin M. 2021. Allergome-wide peptide microarrays enable epitope deconvolution in allergen-specific immunotherapy. Journal of Allergy and Clinical Immunology. 147(3):1077-1086. https://doi.org/10.1016/j.jaci.2020.08.002

MLA

Vancouver

Author

Mikus, Maria ; Zandian, Arash ; Sjöberg, Ronald ; Hamsten, Carl ; Forsström, Björn ; Andersson, Morgan ; Greiff, Lennart ; Uhlén, Mathias ; Levin, Mattias ; Nilsson, Peter ; van Hage, Marianne ; Ohlin, Mats. / Allergome-wide peptide microarrays enable epitope deconvolution in allergen-specific immunotherapy. I: Journal of Allergy and Clinical Immunology. 2021 ; Vol. 147, Nr. 3. s. 1077-1086.

RIS

TY - JOUR

T1 - Allergome-wide peptide microarrays enable epitope deconvolution in allergen-specific immunotherapy

AU - Mikus, Maria

AU - Zandian, Arash

AU - Sjöberg, Ronald

AU - Hamsten, Carl

AU - Forsström, Björn

AU - Andersson, Morgan

AU - Greiff, Lennart

AU - Uhlén, Mathias

AU - Levin, Mattias

AU - Nilsson, Peter

AU - van Hage, Marianne

AU - Ohlin, Mats

PY - 2021

Y1 - 2021

N2 - Background: The interaction of allergens and allergen-specific IgE initiates the allergic cascade after crosslinking of receptors on effector cells. Antibodies of other isotypes may modulate such a reaction. Receptor crosslinking requires binding of antibodies to multiple epitopes on the allergen. Limited information is available on the complexity of the epitope structure of most allergens. Objectives: We sought to allow description of the complexity of IgE, IgG4, and IgG epitope recognition at a global, allergome-wide level during allergen-specific immunotherapy (AIT). Methods: We generated an allergome-wide microarray comprising 731 allergens in the form of more than 172,000 overlapping 16-mer peptides. Allergen recognition by IgE, IgG4, and IgG was examined in serum samples collected from subjects undergoing AIT against pollen allergy. Results: Extensive induction of linear peptide-specific Phl p 1– and Bet v 1–specific humoral immunity was demonstrated in subjects undergoing a 3-year-long AIT against grass and birch pollen allergy, respectively. Epitope profiles differed between subjects but were largely established already after 1 year of AIT, suggesting that dominant allergen-specific antibody clones remained as important contributors to humoral immunity following their initial establishment during the early phase of AIT. Complex, subject-specific patterns of allergen isoform and group cross-reactivities in the repertoires were observed, patterns that may indicate different levels of protection against different allergen sources. Conclusions: The study highlights the complexity and subject-specific nature of allergen epitopes recognized following AIT. We envisage that epitope deconvolution will be an important aspect of future efforts to describe and analyze the outcomes of AIT in a personalized manner.

AB - Background: The interaction of allergens and allergen-specific IgE initiates the allergic cascade after crosslinking of receptors on effector cells. Antibodies of other isotypes may modulate such a reaction. Receptor crosslinking requires binding of antibodies to multiple epitopes on the allergen. Limited information is available on the complexity of the epitope structure of most allergens. Objectives: We sought to allow description of the complexity of IgE, IgG4, and IgG epitope recognition at a global, allergome-wide level during allergen-specific immunotherapy (AIT). Methods: We generated an allergome-wide microarray comprising 731 allergens in the form of more than 172,000 overlapping 16-mer peptides. Allergen recognition by IgE, IgG4, and IgG was examined in serum samples collected from subjects undergoing AIT against pollen allergy. Results: Extensive induction of linear peptide-specific Phl p 1– and Bet v 1–specific humoral immunity was demonstrated in subjects undergoing a 3-year-long AIT against grass and birch pollen allergy, respectively. Epitope profiles differed between subjects but were largely established already after 1 year of AIT, suggesting that dominant allergen-specific antibody clones remained as important contributors to humoral immunity following their initial establishment during the early phase of AIT. Complex, subject-specific patterns of allergen isoform and group cross-reactivities in the repertoires were observed, patterns that may indicate different levels of protection against different allergen sources. Conclusions: The study highlights the complexity and subject-specific nature of allergen epitopes recognized following AIT. We envisage that epitope deconvolution will be an important aspect of future efforts to describe and analyze the outcomes of AIT in a personalized manner.

KW - Allergen

KW - allergen-specific immunotherapy

KW - antibody

KW - epitope

KW - IgE

KW - IgG

KW - linear epitope

KW - peptide microarray

U2 - 10.1016/j.jaci.2020.08.002

DO - 10.1016/j.jaci.2020.08.002

M3 - Article

C2 - 32791163

AN - SCOPUS:85091214366

VL - 147

SP - 1077

EP - 1086

JO - Journal of Allergy and Clinical Immunology

JF - Journal of Allergy and Clinical Immunology

SN - 1097-6825

IS - 3

ER -