Alternative oxidase-mediated respiration prevents lethal mitochondrial cardiomyopathy

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Alternative oxidase (AOX) is a non-mammalian enzyme that can bypass blockade of the complex III-IV segment of the respiratory chain (RC). We crossed a Ciona intestinalis AOX transgene into RC complex III (cIII)-deficient Bcs1lp.S78G knock-in mice, displaying multiple visceral manifestations and premature death. The homozygotes expressing AOX were viable, and their median survival was extended from 210 to 590 days due to permanent prevention of lethal cardiomyopathy. AOX also prevented renal tubular atrophy and cerebral astrogliosis, but not liver disease, growth restriction, or lipodystrophy, suggesting distinct tissue-specific pathogenetic mechanisms. Assessment of reactive oxygen species (ROS) production and damage suggested that ROS were not instrumental in the rescue. Cardiac mitochondrial ultrastructure, mitochondrial respiration, and pathological transcriptome and metabolome alterations were essentially normalized by AOX, showing that the restored electron flow upstream of cIII was sufficient to prevent cardiac energetic crisis and detrimental decompensation. These findings demonstrate the value of AOX, both as a mechanistic tool and a potential therapeutic strategy, for cIII deficiencies.

Detaljer

Författare
  • Jayasimman Rajendran
  • Janne Purhonen
  • Saara Tegelberg
  • Olli Pekka Smolander
  • Matthias Mörgelin
  • Jan Rozman
  • Valerie Gailus-Durner
  • Helmut Fuchs
  • Martin Hrabe de Angelis
  • Petri Auvinen
  • Eero Mervaala
  • Howard T. Jacobs
  • Marten Szibor
  • Vineta Fellman
  • Jukka Kallijärvi
Enheter & grupper
Externa organisationer
  • Folkhälsan Research Centre
  • University of Helsinki
  • Helmholtz Zentrum München
  • German Center for Diabetes Research
  • Technical University of Munich
  • University of Tampere
  • Helsinki University Central Hospital
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Fysiologi

Nyckelord

Originalspråkengelska
Artikelnummere9456
TidskriftEMBO Molecular Medicine
Volym2018
StatusPublished - 2018
PublikationskategoriForskning
Peer review utfördJa