β-amyloid peptides inhibit acetylcholine release from cholinergic presynaptic nerve endings isolated from an electric ray

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

We investigated the effects of β-amyloid (Aβ) peptides on cholinergic synaptosomes isolated from the electric organ of the Japanese marine ray Narke japonica. Fresh and pre-incubated solutions of Aβ(1-42) inhibited acetylcholine (ACh) release from the synaptosomes evoked by high [K+] depolarization when incubated with synaptosomes for 10 min before the depolarizing stimulus. A freshly prepared solution of Aβ(1-40) did not inhibit the evoked ACh release, but prolonged pre-incubation of Aβ(1-40) solution caused the inhibition. Aβ(1-15) neither in fresh nor pre-incubated solution inhibited. These results have demonstrated that Aβ peptides can acutely inhibit the depolarization-evoked release of ACh by acting directly on cholinergic presynaptic nerve endings. The electrophoresis analysis showed a strong correlation between Aβ aggregation and its inhibition for ACh release.

Detaljer

Författare
  • Yasushi Satoh
  • Yutaka Hirakura
  • Sotaro Shibayama
  • Naohide Hirashima
  • Toshiharu Suzuki
  • Yutaka Kirino
Externa organisationer
  • University of Tokyo
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Neurovetenskaper

Nyckelord

Originalspråkengelska
Sidor (från-till)97-100
Antal sidor4
TidskriftNeuroscience Letters
Volym302
Utgåva nummer2-3
StatusPublished - 2001 apr 20
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa