β-amyloid peptides inhibit acetylcholine release from cholinergic presynaptic nerve endings isolated from an electric ray

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β-amyloid peptides inhibit acetylcholine release from cholinergic presynaptic nerve endings isolated from an electric ray. / Satoh, Yasushi; Hirakura, Yutaka; Shibayama, Sotaro; Hirashima, Naohide; Suzuki, Toshiharu; Kirino, Yutaka.

I: Neuroscience Letters, Vol. 302, Nr. 2-3, 20.04.2001, s. 97-100.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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Satoh, Yasushi ; Hirakura, Yutaka ; Shibayama, Sotaro ; Hirashima, Naohide ; Suzuki, Toshiharu ; Kirino, Yutaka. / β-amyloid peptides inhibit acetylcholine release from cholinergic presynaptic nerve endings isolated from an electric ray. I: Neuroscience Letters. 2001 ; Vol. 302, Nr. 2-3. s. 97-100.

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TY - JOUR

T1 - β-amyloid peptides inhibit acetylcholine release from cholinergic presynaptic nerve endings isolated from an electric ray

AU - Satoh, Yasushi

AU - Hirakura, Yutaka

AU - Shibayama, Sotaro

AU - Hirashima, Naohide

AU - Suzuki, Toshiharu

AU - Kirino, Yutaka

PY - 2001/4/20

Y1 - 2001/4/20

N2 - We investigated the effects of β-amyloid (Aβ) peptides on cholinergic synaptosomes isolated from the electric organ of the Japanese marine ray Narke japonica. Fresh and pre-incubated solutions of Aβ(1-42) inhibited acetylcholine (ACh) release from the synaptosomes evoked by high [K+] depolarization when incubated with synaptosomes for 10 min before the depolarizing stimulus. A freshly prepared solution of Aβ(1-40) did not inhibit the evoked ACh release, but prolonged pre-incubation of Aβ(1-40) solution caused the inhibition. Aβ(1-15) neither in fresh nor pre-incubated solution inhibited. These results have demonstrated that Aβ peptides can acutely inhibit the depolarization-evoked release of ACh by acting directly on cholinergic presynaptic nerve endings. The electrophoresis analysis showed a strong correlation between Aβ aggregation and its inhibition for ACh release.

AB - We investigated the effects of β-amyloid (Aβ) peptides on cholinergic synaptosomes isolated from the electric organ of the Japanese marine ray Narke japonica. Fresh and pre-incubated solutions of Aβ(1-42) inhibited acetylcholine (ACh) release from the synaptosomes evoked by high [K+] depolarization when incubated with synaptosomes for 10 min before the depolarizing stimulus. A freshly prepared solution of Aβ(1-40) did not inhibit the evoked ACh release, but prolonged pre-incubation of Aβ(1-40) solution caused the inhibition. Aβ(1-15) neither in fresh nor pre-incubated solution inhibited. These results have demonstrated that Aβ peptides can acutely inhibit the depolarization-evoked release of ACh by acting directly on cholinergic presynaptic nerve endings. The electrophoresis analysis showed a strong correlation between Aβ aggregation and its inhibition for ACh release.

KW - β-Amyloid

KW - aggregation

KW - cholinergic neurons

KW - electric organ

KW - high [K] depolarization

KW - nerve terminals

KW - synaptosomes

UR - http://www.scopus.com/inward/record.url?scp=0035917827&partnerID=8YFLogxK

U2 - 10.1016/S0304-3940(01)01665-2

DO - 10.1016/S0304-3940(01)01665-2

M3 - Article

C2 - 11290396

AN - SCOPUS:0035917827

VL - 302

SP - 97

EP - 100

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 2-3

ER -