An AC Electrokinetic Device for the rapid separation and detection of cancer related DNA nanoparticulate biomarkers

Forskningsoutput: Kapitel i bok/rapport/Conference proceedingKonferenspaper i proceeding

Abstract

The ability to rapidly detect cell free circulating (cfc) DNA and other nanoparticulate disease biomarkers directly in blood is a major challenge for nanomedicine. We now show that AC Electrokinetic (ACE) microelectrode array devices can be used to rapidly isolate and detect cfc-DNA nanoparticulates directly from whole blood and other high conductance samples (plasma, serum etc.). The device shows isolation of naked DNA in water and plasma at >500bp size and can isolate DNA down to 50 picograms in 50uL of fluid. Disease specific cfc-DNA materials could also be detected directly in blood from patients with Chronic Lymphocytic Leukemia.

Detaljer

Författare
  • Rajaram Krishnan
  • David Charlot
  • Lucas Kumosa
  • William Hanna
  • Jerry Lu
  • Avery Sonnenberg
  • Michael Heller
Externa organisationer
  • Biological Dynamics, Inc.
  • University of California, San Diego
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biomedicinsk laboratorievetenskap/teknologi
Originalspråkengelska
Titel på värdpublikationBiomedical Circuits and Systems Conference (BioCAS), 2011 IEEE
UtgivningsortSan Diego
Sidor373-376
StatusPublished - 2011
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa
Evenemang2011 IEEE Biomedical Circuits and Systems Conference, BioCAS 2011 - San Diego, CA, USA
Varaktighet: 2011 nov 102011 nov 12

Konferens

Konferens2011 IEEE Biomedical Circuits and Systems Conference, BioCAS 2011
LandUSA
OrtSan Diego, CA
Period2011/11/102011/11/12