An alternative method for calibration of flow field flow fractionation channels for hydrodynamic radius determination: The nanoemulsion method (featuring multi angle light scattering)

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Abstract

This study suggests a novel method for determination of the channel height in asymmetrical flow field-flow fractionation (AF4), which can be used for calibration of the channel for hydrodynamic radius determinations. The novel method uses an oil-in-water nanoemulsion together with multi angle light scattering (MALS) and elution theory to determine channel height from an AF4 experiment. The method is validated using two orthogonal methods; first, by using standard particle elution experiments and, secondly, by imaging an assembled and carrier liquid filled channel by x-ray computed tomography (XCT). It is concluded that the channel height can be determined with approximately the same accuracy as with the traditional channel height determination technique. However, the nanoemulsion method can be used under more challenging conditions than standard particles, as the nanoemulsion remains stable in a wider pH range than the previously used standard particles. Moreover, the novel method is also more cost effective.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • Kristianstad University
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Strömningsmekanik och akustik

Nyckelord

Originalspråkengelska
Sidor (från-till)155-163
Antal sidor9
TidskriftJournal of Chromatography A
Volym1533
StatusPublished - 2018 jan 19
PublikationskategoriForskning
Peer review utfördJa

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