An amperometric biosensor based on laccase immobilized in polymer matrices for determining phenolic compounds

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An amperometric biosensor based on laccase immobilized in polymer matrices for determining phenolic compounds. / Yaropolov, A I; Shleev, Sergey; Morozova, O V; Zaitseva, E A; Marko-Varga, G; Emnéus, Jenny; Gorton, Lo.

I: Journal of Analytical Chemistry, Vol. 60, Nr. 6, 2005, s. 553-557.

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Yaropolov, A I ; Shleev, Sergey ; Morozova, O V ; Zaitseva, E A ; Marko-Varga, G ; Emnéus, Jenny ; Gorton, Lo. / An amperometric biosensor based on laccase immobilized in polymer matrices for determining phenolic compounds. I: Journal of Analytical Chemistry. 2005 ; Vol. 60, Nr. 6. s. 553-557.

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TY - JOUR

T1 - An amperometric biosensor based on laccase immobilized in polymer matrices for determining phenolic compounds

AU - Yaropolov, A I

AU - Shleev, Sergey

AU - Morozova, O V

AU - Zaitseva, E A

AU - Marko-Varga, G

AU - Emnéus, Jenny

AU - Gorton, Lo

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)

PY - 2005

Y1 - 2005

N2 - An amperometric enzyme electrode based on laccase for determining phenolic compounds is proposed. The following three types of polymer materials were used for enzyme immobilization on the surface of a glassy-carbon electrode: positively charged cetyl ethyl poly (ethyleneimine) (CEPEI) and negatively charged commercial Nafion and Eastman AQ 29D polymers. The advantages and disadvantages of each of the above polymers for enzyme immobilization are discussed. The detection limits of the model phenolic compounds hydroquinone and pyrocatechol in a buffer solution on laccase immobilization in a Nation membrane were 3.5 x 10(-8) and 5.0 x 10(-8) M, respectively, at a signal-to-noise ratio of 3. Electrodes with laccase immobilized in Nation and Eastman AQ 29D membranes exhibited the shortest response time. The operating stability and the stability in storage can be significantly improved by the additional incorporation of gelatin in the polymer matrices. Gelatin prevents enzyme inactivation as a result of enzyme modification by the free-radical oxidation products of phenolic compounds.

AB - An amperometric enzyme electrode based on laccase for determining phenolic compounds is proposed. The following three types of polymer materials were used for enzyme immobilization on the surface of a glassy-carbon electrode: positively charged cetyl ethyl poly (ethyleneimine) (CEPEI) and negatively charged commercial Nafion and Eastman AQ 29D polymers. The advantages and disadvantages of each of the above polymers for enzyme immobilization are discussed. The detection limits of the model phenolic compounds hydroquinone and pyrocatechol in a buffer solution on laccase immobilization in a Nation membrane were 3.5 x 10(-8) and 5.0 x 10(-8) M, respectively, at a signal-to-noise ratio of 3. Electrodes with laccase immobilized in Nation and Eastman AQ 29D membranes exhibited the shortest response time. The operating stability and the stability in storage can be significantly improved by the additional incorporation of gelatin in the polymer matrices. Gelatin prevents enzyme inactivation as a result of enzyme modification by the free-radical oxidation products of phenolic compounds.

U2 - 10.1007/s10809-005-0138-2

DO - 10.1007/s10809-005-0138-2

M3 - Article

VL - 60

SP - 553

EP - 557

JO - Journal of Analytical Chemistry

JF - Journal of Analytical Chemistry

SN - 1608-3199

IS - 6

ER -