An ELISA for grancalcin, a novel cytosolic calcium-binding protein present in leukocytes

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Grancalcin is a newly discovered cytosolic calcium-binding protein, belonging to the group of EF-hand proteins. Grancalcin is specifically associated with cells originating in the bone marrow. Grancalcin binds reversibly to secretory vesicles and plasma membranes in human neutrophils and might therefore play a role in the regulation of vesicle/granule exocytosis. We describe here the production of recombinant grancalcin, the generation of antibodies to the protein and the development of a specific, accurate and sensitive ELISA for the detection of human leukocytic grancalcin. This ELISA may be useful for monitoring leukocyte infiltration into tissues.

Detaljer

Författare
  • K Lollike
  • O Sørensen
  • J R Bundgaard
  • A W Segal
  • A Boyhan
  • N Borregaard
Externa organisationer
  • Copenhagen University Hospital
  • University College London
Forskningsområden

Nyckelord

Originalspråkengelska
Sidor (från-till)1-8
Antal sidor8
TidskriftJournal of Immunological Methods
Volym185
Utgåva nummer1
StatusPublished - 1995 sep 11
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa