An Improvement on MS-based Epigenetic Analysis of Large Histone-derived Peptides by Using the IVU Interface.
Forskningsoutput: Tidskriftsbidrag › Artikel i vetenskaplig tidskrift
Highly protonated histone-derived peptides impede a sufficient mass spectrometry (MS) -based epigenetic analysis since their relatively low m/z due to a high degree of proton addition to peptides would difficult to analyze the resulting complex MS/MS spectra. In order to reduce the degree of protonations, we have developed a new interface, the IVU, in which peptides are ionized under a vaporized organic solvent, and it has demonstrated that the doubly-charged histone-tail H2B peptide, PEPAKSAPAPKKGSKKAVTKAQKK (m/z 1238.243 , +2), has been detected by using the IVU interface and sequenced, which was not detectable before.
|Enheter & grupper|
Ämnesklassifikation (UKÄ) – OBLIGATORISK
|Utgåva nummer||Online 05 June 2015|
|Status||Published - 2015|
|Peer review utförd||Ja|