An Improvement on MS-based Epigenetic Analysis of Large Histone-derived Peptides by Using the IVU Interface.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Highly protonated histone-derived peptides impede a sufficient mass spectrometry (MS) -based epigenetic analysis since their relatively low m/z due to a high degree of proton addition to peptides would difficult to analyze the resulting complex MS/MS spectra. In order to reduce the degree of protonations, we have developed a new interface, the IVU, in which peptides are ionized under a vaporized organic solvent, and it has demonstrated that the doubly-charged histone-tail H2B peptide, PEPAKSAPAPKKGSKKAVTKAQKK (m/z 1238.243 , +2), has been detected by using the IVU interface and sequenced, which was not detectable before.

Detaljer

Författare
  • Tetsuya Fukuda
  • Hiroshi Hike
  • Fumihiko Usui
  • Yasuhiko Bando
  • Toshide Nishimura
  • Tatsuhiko Kodama
  • Takeshi Kawamura
Enheter & grupper
Externa organisationer
  • Biosys Technologies, Inc
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Analytisk kemi
  • Läkemedelskemi
Originalspråkengelska
Sidor (från-till)14-16
TidskriftAnalytical Biochemistry
Volym486
Utgåva nummerOnline 05 June 2015
StatusPublished - 2015
PublikationskategoriForskning
Peer review utfördJa