Application of in-house virtual protein database performed in genomic-proteomic combined research on heavy-metal stressed onion roots

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Objectives To establish an in-house virtual protein database that can be employed in proteomic research on non-model plants. Results A total of 87,430 unigenes were obtained through transcriptome sequencing from onion roots. Of these, 24,305 unigenes were annotated and their nucleotide sequences of coding regions were translated into amino acid sequences. The corresponding 24,305 amino acid sequences were considered as an in-house virtual protein database. Thirty-two protein spots with significant differential abundance were selected. Their MS data were submitted to a restriction enzyme map which was converted from the in-house virtual protein database. A total of 27 proteins were finally matched.
Conclusions The in-house protein database is a
feasible and innovative strategy for proteomic
research on non-model plants.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • South China Normal University
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biokemi och molekylärbiologi

Nyckelord

Originalspråkengelska
Sidor (från-till)1293-1300
Antal sidor8
TidskriftBiotechnology Letters
Volym38
Utgivningsnummer8
Tidigt onlinedatum2016
StatusPublished - 2016
PublikationskategoriForskning
Peer review utfördJa