Aquaporin-4-dependent glymphatic solute transport in the rodent brain

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift


title = "Aquaporin-4-dependent glymphatic solute transport in the rodent brain",
abstract = "The glymphatic system is a brain-wide clearance pathway; its impairment contributes to the accumulation of amyloid-β. Influx of cerebrospinal fluid (CSF) depends upon the expression and perivascular localization of the astroglial water channel aquaporin-4 (AQP4). Prompted by a recent failure to find an effect of Aqp4 knock-out (KO) on CSF and interstitial fluid (ISF) tracer transport, five groups re-examined the importance of AQP4 in glymphatic transport. We concur that CSF influx is higher in wild-type mice than in four different Aqp4 KO lines and in one line that lacks perivascular AQP4 (Snta1 KO). Meta-analysis of all studies demonstrated a significant decrease in tracer transport in KO mice and rats compared to controls. Meta-regression indicated that anesthesia, age, and tracer delivery explain the opposing results. We also report that intrastriatal injections suppress glymphatic function. This validates the role of AQP4 and shows that glymphatic studies must avoid the use of invasive procedures.",
keywords = "aquaporin-4, cerebrospinal fluid, glymphatic, meta-analysis, mouse, neuroscience, replication study, solute transport",
author = "Humberto Mestre and Hablitz, {Lauren M.} and Xavier, {Anna Lr} and Weixi Feng and Wenyan Zou and Tinglin Pu and Hiromu Monai and Giridhar Murlidharan and {Castellanos Rivera}, {Ruth M.} and Simon, {Matthew J.} and Pike, {Martin M.} and Virginia Pl{\'a} and Ting Du and Kress, {Benjamin T.} and Xiaowen Wang and Plog, {Benjamin A.} and Thrane, {Alexander S.} and Iben Lundgaard and Yoichiro Abe and Masato Yasui and Thomas, {John H.} and Ming Xiao and Hajime Hirase and Aravind Asokan and Iliff, {Jeffrey J.} and Maiken Nedergaard",
year = "2018",
month = "12",
day = "18",
doi = "10.7554/eLife.40070",
language = "English",
volume = "7",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications LTD.",