Aqueous two-phase partitioning for proteomic monitoring of cell surface biomarkers in human peripheral blood mononuclear cells

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Bibtex

@article{b5c723a2bfbb4a848e3970ac83a5e84d,
title = "Aqueous two-phase partitioning for proteomic monitoring of cell surface biomarkers in human peripheral blood mononuclear cells",
abstract = "For proteomic monitoring of processes such as allergy or inflammation an efficient pre-fractionation strategy is required. We isolated plasma membranes from human peripheral blood mononuclear (PBM) cells by aqueous two-phase partitioning. After 1DE combined with LC-MS/MS, several cell surface marker proteins and in total 60 different plasma membrane proteins (out of 84 identified proteins, i.e., 72{\%}) were detected. Plasma membranes obtained were from only one human donor, the procedure is therefore applicable for individual patient screening.",
keywords = "proteomics, prefractionation, two-phase partitioning, aqueous, cell surface biomarkers, allergy, plasma membrane proteins",
author = "Henrik Everberg and {Peterson Wulff}, Ragna and S Rak and Folke Tjerneld and Cecilia Emanuelsson",
year = "2006",
doi = "10.1021/pr050469z",
language = "English",
volume = "5",
pages = "1168--1175",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "The American Chemical Society (ACS)",
number = "5",

}