Aqueous two-phase partitioning for proteomic monitoring of cell surface biomarkers in human peripheral blood mononuclear cells

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Aqueous two-phase partitioning for proteomic monitoring of cell surface biomarkers in human peripheral blood mononuclear cells. / Everberg, Henrik; Peterson Wulff, Ragna; Rak, S; Tjerneld, Folke; Emanuelsson, Cecilia.

I: Journal of Proteome Research, Vol. 5, Nr. 5, 2006, s. 1168-1175.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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T1 - Aqueous two-phase partitioning for proteomic monitoring of cell surface biomarkers in human peripheral blood mononuclear cells

AU - Everberg, Henrik

AU - Peterson Wulff, Ragna

AU - Rak, S

AU - Tjerneld, Folke

AU - Emanuelsson, Cecilia

PY - 2006

Y1 - 2006

N2 - For proteomic monitoring of processes such as allergy or inflammation an efficient pre-fractionation strategy is required. We isolated plasma membranes from human peripheral blood mononuclear (PBM) cells by aqueous two-phase partitioning. After 1DE combined with LC-MS/MS, several cell surface marker proteins and in total 60 different plasma membrane proteins (out of 84 identified proteins, i.e., 72%) were detected. Plasma membranes obtained were from only one human donor, the procedure is therefore applicable for individual patient screening.

AB - For proteomic monitoring of processes such as allergy or inflammation an efficient pre-fractionation strategy is required. We isolated plasma membranes from human peripheral blood mononuclear (PBM) cells by aqueous two-phase partitioning. After 1DE combined with LC-MS/MS, several cell surface marker proteins and in total 60 different plasma membrane proteins (out of 84 identified proteins, i.e., 72%) were detected. Plasma membranes obtained were from only one human donor, the procedure is therefore applicable for individual patient screening.

KW - proteomics

KW - prefractionation

KW - two-phase partitioning

KW - aqueous

KW - cell surface biomarkers

KW - allergy

KW - plasma membrane proteins

U2 - 10.1021/pr050469z

DO - 10.1021/pr050469z

M3 - Article

VL - 5

SP - 1168

EP - 1175

JO - Journal of Proteome Research

T2 - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 5

ER -