Association of tapasin and COPI provides a mechanism for the retrograde transport of major histocompatibility complex (MHC) class I molecules from the Golgi complex to the endoplasmic reticulum

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Bibtex

@article{85102695807b4022b51b54eec818806c,
title = "Association of tapasin and COPI provides a mechanism for the retrograde transport of major histocompatibility complex (MHC) class I molecules from the Golgi complex to the endoplasmic reticulum",
abstract = "Tapasin is a subunit of the transporter associated with antigen processing (TAP). It associates with the major histocompatibility complex (MHC) class I. We show that tapasin interacts with beta- and gamma-subunits of COPI coatomer. COPI retrieves membrane proteins from the Golgi network back to the endoplasmic reticulum (ER). The COPI subunit-associated tapasin also interacts with MHC class I molecules suggesting that tapasin acts as the cargo receptor for packing MHC class I molecules as cargo proteins into COPI-coated vesicles. In tapasin mutant cells, neither TAP nor MHC class I are detected in association with the COPI coatomer. Interestingly, tapasin-associated MHC class I molecules are antigenic peptide-receptive and detected in both the ER and the Golgi. Our data suggest that tapasin is required for the COPI vesicle-mediated retrograde transport of immature MHC class I molecules from the Golgi network to the ER.",
keywords = "Non-U.S. Gov't, Subcellular Fractions/metabolism, Support, Histocompatibility Antigens Class I/*metabolism, Immunoglobulins/*metabolism, Golgi Apparatus/*metabolism, Endoplasmic Reticulum/*metabolism, Coat Protein Complex I/*metabolism, Cell Line, Antiporters/*metabolism, Biological Transport",
author = "Paulsson, {Kajsa M} and Kleijmeer, {Monique J} and Janice Griffith and Marc Jevon and Shangwu Chen and Anderson, {Per O} and Sj{\"o}gren, {Hans Olov} and Su-Ling Li and Ping Wang",
year = "2002",
month = may,
day = "24",
doi = "10.1074/jbc.M201388200",
language = "English",
volume = "277",
pages = "18266--18271",
journal = "Journal of Biological Chemistry",
issn = "1083-351X",
publisher = "ASBMB",
number = "21",

}