Attainment of brown adipocyte features in white adipocytes of hormone-sensitive lipase null mice.

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Attainment of brown adipocyte features in white adipocytes of hormone-sensitive lipase null mice. / Ström, Kristoffer; Hansson, Ola; Lucas, Stephanie; Nevsten, Pernilla; Fernandez, Celine; Klint, Cecilia; Movérare-Skrtic, Sofia; Sundler, Frank; Ohlsson, Claes; Holm, Cecilia.

I: PLoS ONE, Vol. 3, Nr. 3, e1793, 2008.

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Ström, Kristoffer ; Hansson, Ola ; Lucas, Stephanie ; Nevsten, Pernilla ; Fernandez, Celine ; Klint, Cecilia ; Movérare-Skrtic, Sofia ; Sundler, Frank ; Ohlsson, Claes ; Holm, Cecilia. / Attainment of brown adipocyte features in white adipocytes of hormone-sensitive lipase null mice. I: PLoS ONE. 2008 ; Vol. 3, Nr. 3.

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TY - JOUR

T1 - Attainment of brown adipocyte features in white adipocytes of hormone-sensitive lipase null mice.

AU - Ström, Kristoffer

AU - Hansson, Ola

AU - Lucas, Stephanie

AU - Nevsten, Pernilla

AU - Fernandez, Celine

AU - Klint, Cecilia

AU - Movérare-Skrtic, Sofia

AU - Sundler, Frank

AU - Ohlsson, Claes

AU - Holm, Cecilia

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Polymer and Materials Chemistry (LTH) (011001041), Diabetes and Endocrinology (013241530), Neuroendocrine Cell Biology (013212008), Molecular Endocrinology (013212018)

PY - 2008

Y1 - 2008

N2 - BACKGROUND: Hormone-sensitive lipase (HSL) is expressed predominantly in adipose tissue, where it plays an important role in catecholamine-stimulated hydrolysis of stored tri- and diglycerides, thus mobilizing fatty acids. HSL exhibits broad substrate specificity and besides acylglycerides it hydrolyzes cholesteryl esters, retinyl esters and lipoidal esters. Despite its role in fatty acid mobilization, HSL null mice have been shown to be resistant to diet-induced obesity. METHODOLOGY/PRINCIPAL FINDINGS: Following a high-fat diet (HFD) regimen, energy expenditure, measured using indirect calorimetry, was increased in HSL null mice. White adipose tissue of HSL null mice was characterized by reduced mass and reduced protein expression of PPARgamma, a key transcription factor in adipogenesis, and stearoyl-CoA desaturase 1, the expression of which is known to be positively correlated to the differentiation state of the adipocyte. The protein expression of uncoupling protein-1 (UCP-1), the highly specific marker of brown adipocytes, was increased 7-fold in white adipose tissue of HSL null mice compared to wildtype littermates. Transmission electron microscopy revealed an increase in the size of mitochondria of white adipocytes of HSL null mice. The mRNA expression of pRb and RIP140 was decreased in isolated white adipocytes, while the expression of UCP-1 and CPT1 was increased in HSL null mice compared to wildtype littermates. Basal oxygen consumption was increased almost 3-fold in white adipose tissue of HSL null mice and was accompanied by increased uncoupling activity. CONCLUSIONS: These data suggest that HSL is involved in the determination of white versus brown adipocytes during adipocyte differentiation The exact mechanism(s) underlying this novel role of HSL remains to be elucidated, but it seems clear that HSL is required to sustain normal expression levels of pRb and RIP140, which both promote differentiation into the white, rather than the brown, adipocyte lineage.

AB - BACKGROUND: Hormone-sensitive lipase (HSL) is expressed predominantly in adipose tissue, where it plays an important role in catecholamine-stimulated hydrolysis of stored tri- and diglycerides, thus mobilizing fatty acids. HSL exhibits broad substrate specificity and besides acylglycerides it hydrolyzes cholesteryl esters, retinyl esters and lipoidal esters. Despite its role in fatty acid mobilization, HSL null mice have been shown to be resistant to diet-induced obesity. METHODOLOGY/PRINCIPAL FINDINGS: Following a high-fat diet (HFD) regimen, energy expenditure, measured using indirect calorimetry, was increased in HSL null mice. White adipose tissue of HSL null mice was characterized by reduced mass and reduced protein expression of PPARgamma, a key transcription factor in adipogenesis, and stearoyl-CoA desaturase 1, the expression of which is known to be positively correlated to the differentiation state of the adipocyte. The protein expression of uncoupling protein-1 (UCP-1), the highly specific marker of brown adipocytes, was increased 7-fold in white adipose tissue of HSL null mice compared to wildtype littermates. Transmission electron microscopy revealed an increase in the size of mitochondria of white adipocytes of HSL null mice. The mRNA expression of pRb and RIP140 was decreased in isolated white adipocytes, while the expression of UCP-1 and CPT1 was increased in HSL null mice compared to wildtype littermates. Basal oxygen consumption was increased almost 3-fold in white adipose tissue of HSL null mice and was accompanied by increased uncoupling activity. CONCLUSIONS: These data suggest that HSL is involved in the determination of white versus brown adipocytes during adipocyte differentiation The exact mechanism(s) underlying this novel role of HSL remains to be elucidated, but it seems clear that HSL is required to sustain normal expression levels of pRb and RIP140, which both promote differentiation into the white, rather than the brown, adipocyte lineage.

U2 - 10.1371/journal.pone.0001793

DO - 10.1371/journal.pone.0001793

M3 - Article

C2 - 18335062

VL - 3

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 3

M1 - e1793

ER -