beta-Microseminoprotein binds CRISP-3 in human seminal plasma

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

P-Microseminoprotein (MSP) and cysteine-rich secretory protein 3 (CRISP-3) are abundant constituents of human seminal plasma. Immunoprecipitation and gel filtration of seminal plasma proteins combined with examination of the proteins in their pure form showed that MSP and CRISP-3 form stable, non-covalent complexes. CRISP-3 binds MSP with very high affinity, as evidenced by surface plasmon resonance. Due to far higher abundance of MSP in prostatic fluid, it manifests large overcapacity for CRISP-3 binding. Structural similarity with an MSP-binding protein from blood plasma suggests that CRISP-3 binds MSP through its ami-terminal SCP-domain.

Detaljer

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Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biologiska vetenskaper

Nyckelord

Originalspråkengelska
Sidor (från-till)555-561
TidskriftBiochemical and Biophysical Research Communications
Volym333
Utgivningsnummer2
StatusPublished - 2005
PublikationskategoriForskning
Peer review utfördJa