Blinking statistics of a molecular beacon triggered by end-denaturation of DNA

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Abstract

We use a master equation approach based on the Poland–Scheraga free energy for DNA denaturation to investigate the (un)zipping dynamics of a denaturation wedge in a stretch of DNA that is clamped at one end. In particular, we quantify the blinking dynamics of a fluorophore–quencher pair mounted within the denaturation wedge. We also study the behavioural changes in the presence of proteins that selectively bind to single-stranded DNA. We show that such a set-up could be well suited as an easy-to-implement nanodevice for sensing environmental conditions in small volumes.

Detaljer

Författare
Externa organisationer
  • Nordic Institute for Theoretical Atomic Physics (NORDITA)
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biofysik
Originalspråkengelska
Sidor (från-till)S4305-S4316
TidskriftJournal of Physics: Condensed Matter
Volym17
Utgivningsnummer49
StatusPublished - 2005 dec 14
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa