Bronchial mucosal mast cells in asymptomatic smokers relation to structure, lung function and emphysema

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Bronchial mucosal mast cells in asymptomatic smokers relation to structure, lung function and emphysema. / Ekberg-Jansson, A; Amin, K; Bake, B; Rosengren, A; Tylen, U; Venge, P; Löfdahl, Claes-Göran.

I: Respiratory Medicine, Vol. 99, Nr. 1, 2005, s. 75-83.

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Ekberg-Jansson, A ; Amin, K ; Bake, B ; Rosengren, A ; Tylen, U ; Venge, P ; Löfdahl, Claes-Göran. / Bronchial mucosal mast cells in asymptomatic smokers relation to structure, lung function and emphysema. I: Respiratory Medicine. 2005 ; Vol. 99, Nr. 1. s. 75-83.

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TY - JOUR

T1 - Bronchial mucosal mast cells in asymptomatic smokers relation to structure, lung function and emphysema

AU - Ekberg-Jansson, A

AU - Amin, K

AU - Bake, B

AU - Rosengren, A

AU - Tylen, U

AU - Venge, P

AU - Löfdahl, Claes-Göran

PY - 2005

Y1 - 2005

N2 - The pathologic mechanisms of chronic obstructive pulmonary disease (COPD) most certainly involves neutrophil granulocytes, cytotoxic T-cells, macophages and mast cells. The aim of this study was to investigate the relation between the number of mast cells in different compartments in bronchial biopsies of central proximal airways to structural changes, lung function tests and emphysema detected by high resolution computed tomography (HRCT). Twenty nine asymptomatic smoking and 16 never-smoking men from a population study were recruited. Central bronchial biopsies were stained to identify mast cells by immunohistochemistry. The number of mast cells in the epithelium, lamina propria and smooth muscle as welt as epithelial integrity and thickness of the tenascin and laminin layer were determined. Smokers had increased numbers of mast cells in all compartments (P<0.001). Structural changes were correlated to mast cell numbers with the closest associations to mast cell numbers in the smooth muscle [epithelial integrity (R-S=-0.48, P=0.008), laminin layer (R-S=0.63, P=0.0002), tenascin layer (R-S=0.40, P=0.03)]. Similar correlations between mast cells and lung function tests were seen [functional residual capacity (FRC) (R-S=0.60, P=0.0006), total lung capacity (TLC) (R-S=0.44, P=0.02) and residual volume (RV) (R-S=0.41, P=0.03)].. No correlations could be detected between mast cells and FEV1 or to emphysema. Smoking is associated with an increase of mast cells in all compartments of the bronchial mucosa, including smooth muscle, and this is related to altered airway structure and function.

AB - The pathologic mechanisms of chronic obstructive pulmonary disease (COPD) most certainly involves neutrophil granulocytes, cytotoxic T-cells, macophages and mast cells. The aim of this study was to investigate the relation between the number of mast cells in different compartments in bronchial biopsies of central proximal airways to structural changes, lung function tests and emphysema detected by high resolution computed tomography (HRCT). Twenty nine asymptomatic smoking and 16 never-smoking men from a population study were recruited. Central bronchial biopsies were stained to identify mast cells by immunohistochemistry. The number of mast cells in the epithelium, lamina propria and smooth muscle as welt as epithelial integrity and thickness of the tenascin and laminin layer were determined. Smokers had increased numbers of mast cells in all compartments (P<0.001). Structural changes were correlated to mast cell numbers with the closest associations to mast cell numbers in the smooth muscle [epithelial integrity (R-S=-0.48, P=0.008), laminin layer (R-S=0.63, P=0.0002), tenascin layer (R-S=0.40, P=0.03)]. Similar correlations between mast cells and lung function tests were seen [functional residual capacity (FRC) (R-S=0.60, P=0.0006), total lung capacity (TLC) (R-S=0.44, P=0.02) and residual volume (RV) (R-S=0.41, P=0.03)].. No correlations could be detected between mast cells and FEV1 or to emphysema. Smoking is associated with an increase of mast cells in all compartments of the bronchial mucosa, including smooth muscle, and this is related to altered airway structure and function.

KW - high-resolution computed

KW - lung function

KW - chronic bronchitis

KW - mast cells

KW - tomography

KW - respiratory symptoms

U2 - 10.1016/j.rmed.2004.05.013

DO - 10.1016/j.rmed.2004.05.013

M3 - Article

VL - 99

SP - 75

EP - 83

JO - Respiratory Medicine

JF - Respiratory Medicine

SN - 1532-3064

IS - 1

ER -